In Vitro Antiviral Activity of Prodelphinidin B-2 3,3′-Di-O-gallate from Myrica rubra

Abstract

In this study, the in vitro antiviral properties of prodelphinidin B-2 3,3′-di-O-gallate (PB233′OG) isolated from the bark of Myrica rubra (Myricaceae) was investigated. Results showed that PB233′OG exhibited anti-herpes simplex virus type 2 (HSV-2) activity with IC₅₀ values of 5.3 ± 0.1 and 0.4 ± 0.04 μM for XTT and plaque reduction assays, respectively. The IC₅₀ value increased with increasing MOI (multiplicity of infection). PB233′OG did not show a cellular cytotoxic effect at concentrations that possessed antiviral activity. Mechanistic studies demonstrated that PB233′OG inhibited HSV-2 attachment to the Vero cell, interfered with the penetration of HSV-2 into the Vero cell, affected the late stage(s) of the HSV-2 infection cycle, and also reduced the viral infectivity at high concentrations. It is concluded that PB233′OG exhibits various modes of action in its anti-HSV-2 effects.

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