Engineering Plants for Elevated CO2: A Relationship between Starch Degradation and Sugar Sensing

T. D. Sharkey; M. Laporte; Y. Lu; S. Weise; A. P. M. Weber

doi:10.1055/s-2004-817911

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2004; 6(3): 280-288
DOI: 10.1055/s-2004-817911

Review Article

Georg Thieme Verlag Stuttgart KG · New York

Engineering Plants for Elevated CO₂: A Relationship between Starch Degradation and Sugar Sensing

T. D. Sharkey¹ , M. Laporte² , Y. Lu¹ , S. Weise¹ , A. P. M. Weber³

¹Department of Botany, University of Wisconsin-Madison, 430 Lincoln Dr., Madison, WI 53706, USA
²Department of Biology, Eastern Michigan University, Ypsilanti, MI 48197, USA
³Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA

Abstract

In the future, plants will have additional CO₂ for photosynthesis. However, plants do not take maximal advantage of this additional CO₂ and it has been hypothesized that end product synthesis limitations and sugar sensing mechanisms are important in regulating plant responses to increasing CO₂. Attempts to increase end product synthesis capacity by engineering increased sucrose-phosphate synthase activity have been generally, but not universally, successful. It was found that plants benefited from a two- to three-fold increase in SPS activity but a 10-fold increase did not increase yield. Despite the success in increasing yield, increasing SPS did not increase photosynthesis. However, carbon export from chloroplasts was increased during the day and reduced at night (when starch provides carbon for sucrose synthesis. We develop here a hypothesis that starch degradation is closely sensed by hexokinase because a newly discovered pathway required for starch to sucrose conversion that involves maltose is one of few metabolic pathways that requires hexokinase activity.

Key words

Genetic engineering - maltose metabolism - photosynthesis - starch breakdown - sugar sensing

Full Text

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T. D. Sharkey

Department of Botany
University of Wisconsin-Madison

430 Lincoln Dr.

Madison, WI 53706

USA

Email: tsharkey@wisc.edu

Guest Editor: F. Loreto