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Thorac Cardiovasc Surg 2004; 52(3): 174-179
DOI: 10.1055/s-2004-817977
Original Thoracic

© Georg Thieme Verlag KG Stuttgart · New York

Warm or Cold Ischemia in Animal Models of Lung Ischemia-Reperfusion Injury: Is There a Difference?

G. Warnecke1 , S. P. Sommer1 , B. Gohrbandt1 , S. Fischer1 , J. M. Hohlfeld2 , J. Niedermeyer2 , A. Haverich1 , M. Strüber1
  • 1Hannover Thoracic Transplant Program, Department of Thoracic and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany
  • 2Hannover Thoracic Transplant Program, Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany
Further Information

Publication History

Received November 19, 2003

Publication Date:
11 June 2004 (online)

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Abstract

Objective: Experiments were designed to compare large animal models for lung ischemia-reperfusion injury using either 90 minutes' warm or 24 hours' cold ischemia. Methods: In 6 pigs, the left lung was perfused in situ with cold LPD solution. Reperfusion was started after 90 min of warm ischemia. The left lung of 6 donor pigs was perfused with LPD solution and the lung transplanted after 24 hours of cold storage into 6 recipient pigs. In both groups the right pulmonary artery and main bronchus were clamped after reperfusion of the left lung. Bronchoalveolar lavage (BAL) was obtained before ischemia and after 2 h of reperfusion. Hemodynamic and respiratory parameters were monitored. Surfactant activity was determined from the BAL in a pulsating bubble surfactometer. Results: Moderate lung injury was evident after reperfusion, but without any significant differences between groups. Surfactant composition and function were mildly impaired after reperfusion in both groups. Conclusions: Our results suggest that short periods of warm ischemia might be accepted as an accelerated model for ischemia-reperfusion injury of the lung after cold storage.

Key words

Ischemia-reperfusion injury - lung transplantation - animal model - LPD solution - warm ischemia

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PD Dr. Martin Strüber

Department of Thoracic and Cardiovascular Surgery
Hannover Medical School

Carl-Neuberg-Straße 1

30623 Hannover

Germany

Phone: + 495115326588

Fax: + 49 51 15 32 84 46

Email: strueber@thg.mh-hannover.de