Four new phenanthroindolizidine alkaloids, tylophoridicines C-F, together with three known ones, R-(+)-deoxytylophorinidine, tylophorinine and tylophorinidine, were isolated from the roots of Tylophora atrofolliculata. The structures were determined on the basis of spectral evidence. These alkaloids exhibited cytotoxic activity in vitro on HCT-8 cell (with IC50 values in the range 0.083 to 18.99 μM) and KB cell (in the range 3.56 to 18.22 μM) lines.
2
Hirokawa M, Yanauchi T, Honda K.
Further investigation of phenanthroindolizidine alkaloids from Tylophora tanakae
.
Chemical and Pharmaceutical Bulletin.
1998;
46
767-9
3
Narasimaha R K, Bhauacharya R K, Venkatachalam S R.
Inhibition of thymidylate synthase and all growth by the phenanthroindolizidine alkaloids.
Cancer Letters.
1998;
128
183-8
5
Chen J J, Duh C Y, Chen I S.
New tetrahydroprotoberberine N-oxide alkaloids and cytotoxic constituents of Corydalis tashiroi
.
Planta Medica.
1999;
65
643-7
6
Mosmann T.
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays.
Journal of Immunological Methods.
1983;
65
55-63
13
Govindachari T R, Viswanathan N, Radhakrishnan J.
Minor alkaloids of Tylophora asthmatica revised structure of tylophorinidine.
Tetrahedron.
1973;
29
891-7
15
Gupta R S, Krepinsky J J, Siminovitch L.
Structural determinants responsible for the biological activity of (-)-emetine, (-)-cryptopleurine, and (-)-tylocrebrine: Structure-activity relationship among related compounds.
Molecular Pharmacology.
1980;
18
136-43
