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Plant Biol (Stuttg) 2004; 6(3): 333-341
DOI: 10.1055/s-2004-820877
Original Paper

Georg Thieme Verlag Stuttgart KG · New York

High-Light Stress and Photoprotection in Umbilicaria antarctica Monitored by Chlorophyll Fluorescence Imaging and Changes in Zeaxanthin and Glutathione

M. Barták1 , J. Hájek1 , H. Vráblíková1 , J. Dubová1
  • 1Masaryk University, Faculty of Science, Department of Plant Physiology and Anatomy, Brno, Czech Republic
Further Information

Publication History

Publication Date:
14 May 2004 (online)

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Abstract

The effect of high light on spatial distribution of chlorophyll (Chl) fluorescence parameters over a lichen thallus (Umbilicaria antarctica) was investigated by imaging of Chl fluorescence parameters before and after exposure to high light (1500 µmol m-2 s-1, 30 min at 5 °C). False colour images of FV/FM and ΦII distribution, taken over thallus with 0.1 mm2 resolution, showed that maximum FV/FM and ΦII values were located close to the thallus centre. Minimum values were typical for thallus margins. After exposure to high light, a differential response of FV/FM and ΦII was found. The marginal thallus part exhibited a loss of photosynthetic activity, manifested as a lack of Chl fluorescence signal, and close-to-centre parts showed a different extent of FV/FM and ΦII decrease. Subsequent recovery in the dark led to a gradual return of FV/FM and ΦII to their initial values. Fast (30 min) and slow (1 - 22 h) phase of recovery were distinguished, suggesting a sufficient capacity of photoprotective mechanisms in U. antarctica to cope with low-temperature photoinhibition. Glutathione and xanthophyll cycle pigments were analyzed by HPLC. High light led to an increase in oxidized glutathione (GSSG), and a conversion of violaxanthin to zeaxanthin, expressed as their de-epoxidation state (DEPS). The responses of GSSG and DEPS were reversible during subsequent recovery in the dark. GSSG and DEPS were highly correlated to non-photochemical quenching (NPQ), indicating involvement of these antioxidants in the resistance of U. antarctica to high-light stress. Heterogeneity of Chl fluorescence parameters over the thallus and differential response to high light are discussed in relation to thallus anatomy and intrathalline distribution of the symbiotic alga Trebouxia sp.

Key words

Antioxidants - lichen - low temperature - oxidative stress - photoinhibition - photosynthesis

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M. Barták

Masaryk University
Faculty of Science
Department of Plant Physiology and Anatomy

Kotlárská 2

61137 Brno

Czech Republic

Email: mbartak@sci.muni.cz

Guest Editor: F. Loreto