Download PDF
Exp Clin Endocrinol Diabetes 2004; 112(7): 364-372
DOI: 10.1055/s-2004-821027
Article

J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Functional Parameters Before and After Parathyroidectomy: A Prospective, Randomized Long-Term Trial On Different Rat Strains

C. Hasse1 , M. Brune1 , W. Lorenz2 , P. Barth3 , W. Metzler4 , G. Breves4 , H. Sitter2
  • 1Department of Visceral, Thoracic and Vascular Surgery, Philipps-University Marburg, Marburg, Germany
  • 2Institute for Theoretical Surgery, Philipps-University Marburg, Marburg, Germany
  • 3Institute for Pathology, Philipps-University Marburg, Marburg, Germany
  • 4Institute for Physiology, University, Hannover, Germany
Further Information

Publication History

Received: June 23, 2003 First decision: August 18, 2003

Accepted: December 8, 2003

Publication Date:
07 July 2004 (online)

Also available at
    Permissions and Reprints

Abstract

For clinical controls before and after parathyroidectomy and for evaluation of the function of transplants of parathyroid tissue, it is necessary to establish standard values of relevant laboratory parameters for donor and recipient animals as well as for different types of nutrition. Since no such data are yet available, it was the purpose to define such standards.

In a prospective randomized trial on 400 rats of the Dark Agouti (DA) and Lewis strain, different functional laboratory parameters such as total calcium, intact parathyroid hormone, phosphate, 1.25-dihydroxyvitamin D, and alkaline phosphatase were measured under a standard and low calcium diet over a period of 40 weeks. Two hundred of these animals underwent a parathyroidectomy four weeks after the beginning of the study and specimens were evaluated histologically.

For all eight different study groups normal values could be defined within tight limits for parameters which describe the function of the parathyroid gland or elements of calcium metabolism under different conditions. The optimal conditions for a transplantation model of parathyroid glands were established. Lewis-rats were identified as the ideal donor and DA rats as the better recipient animals. These data can serve as reference values for future studies on transplantation of the parathyroid without immunosuppression.

Key words

Functional parameters - parathyroidectomy - rats - prospective - randomized long-term trial

References

  • 1 Armbrecht H J, Forte L R. Adaptation of middle age rats to long-term restriction of dietary vitamin D and calcium.  Arch Biochem Biophys. 1985;  242 464-469
    CrossrefPubMedSearch in Google Scholar
  • 2 Armbrecht H J, Forte L R, Halloran B P. Effect of age and dietary calcium on renal (25 OH) D metabolism serum 1.25 (OH)2 D and PTH.  Am J Physiol. 1984;  246 E266-E277
    PubMedSearch in Google Scholar
  • 3 Armbrecht H J, Zenser T V, Gross C J, Davis B B. Adaptation to dietary calcium and phosphorus restriction changes with age in the rat.  Am J Physiol. 1980;  239 E322-E327
    PubMedSearch in Google Scholar
  • 4 Beckman M J, De Luca H F. Regulation of renal vitamin D receptor is an important determination of 1 alpha, 25-dihydroxyvitamin D (3) levels in vivo.  Arch Biochem Biophys. 2002;  401 44-52
    CrossrefPubMedSearch in Google Scholar
  • 5 Bover J, Jara A, Trinidad P, Rodriguez M, Martin-Malo A, Felsenfeld A J. The calcemic response to PTH in the rat: Effect of elevated PTH levels and uremia.  Kidney Int. 1994;  46 310-317
    PubMedSearch in Google Scholar
  • 6 Bronner F. Role of intestinal calcium absorption in plasma calcium regulation of the rat.  Am J Physiol. 1984;  246 116-132
    PubMedSearch in Google Scholar
  • 7 Carditello A. Nodular thyreopathies. Results of 1300 operations.  J Chir Paris. 1990;  127 330
    PubMedSearch in Google Scholar
  • 8 Chan Y-L, Mc Kay C, Dye E, Slatopolsky E. The effect of 1.25 dihydroxycholecalciferol on parathyroid hormone secretion by monolayer cultures of bovine parathyroid cells.  Calcif Tissue Int. 1986;  38 27-32
    PubMedSearch in Google Scholar
  • 9 Demeester-Mirkine N, Hooghe L, Van Geertruyden J, De Maertelaer J. Hypocalcemia after thyroidectomy.  Arch Surg. 1992;  127 854
    CrossrefPubMedSearch in Google Scholar
  • 10 Fox J, Swaminathan R, Murray T M. Role of the parathyroid glands in the enhancement of intestinal calcium absorption in response to a low calcium diet.  J Endocrinol. 1977;  74 345-354
    PubMedSearch in Google Scholar
  • 11 Friedman L M, Furberg C D, DeMets D L. Sample size. Friedman LM, Furberg CD, DeMets DL Fundamentals of Clinical Trials. 2nd ed. Littleton; PSG Publishing Company Inc 1985: 83-107
    Search in Google Scholar
  • 12 Friedman M. Factors influencing parathyroid allotransplantation in rats.  Laryngoscope. 1986;  96 1-16
    PubMedSearch in Google Scholar
  • 13 Hamming J F, Van de Velde C J, Goslings B M, Schelfhout L J, Fleuren G J, Hermans J, Zwaveling A. Prognosis and morbidity after total thyroidectomy for papillary, follicular and medullary thyroid cancer.  Eur J Cancer Clin Oncol. 1989;  25 1317
    CrossrefPubMedSearch in Google Scholar
  • 14 Harris S C. Thyroid and parathyroid surgical complications.  Am J Surg. 1992;  163 476
    CrossrefPubMedSearch in Google Scholar
  • 15 Hasse C, Stinner B, Wagner P K, Rothmund M. Allotransplantation of parathyroid glands in rats: long-term function without immunosuppression.  Langenbecks Arch Chir. 1991;  69 69-74
    PubMedSearch in Google Scholar
  • 16 Larkins R G, MacAuley S J, Colston K W, Evans I M, Galante L S, MacIntyre I. Regulation of vitamin-D metabolism without parathyroid hormone.  Lancet. 1973;  87 289-291
    PubMedSearch in Google Scholar
  • 17 Lempert U G, Scharla S H, Minne H W, Ziegler R. Influence of parathyroidectomy, 1.25-dihydroxyvitamin D3 and high dietary calcium intake on demineralized bone matrix powder-induced bone formation in the rat.  Bone Miner. 1991;  13 103-109
    CrossrefPubMedSearch in Google Scholar
  • 18 Lewin E, Wang W, Olgaard K. Rapid recovery of plasma ionized calcium after induction of hypocalcemia in parathyroidectomized and nephrectomized rats.  Nephrol Dial Transplant. 1998;  14 604-609
    PubMedSearch in Google Scholar
  • 19 Massheimer V, Picotto G, Boland R, De Boland A R. Effect of aging on the mechanisms of PTH-induced calcium influx in rat intestinal cells.  J Cell Physiol. 2000;  1820 429-437
    PubMedSearch in Google Scholar
  • 20 Megherbi M T, Graba A, Abid L, Oulmane D, Saidani M, Benabadij R. Complications and sequelae of benign thyroid surgery.  J Chir Paris. 1992;  129 41
    PubMedSearch in Google Scholar
  • 21 Mulroney S E, Woda C, Haramati A. Changes in renal phosphate reabsorption in the aged rat.  Proc Soc Exp Biol Med. 1998;  218 62-67
    PubMedSearch in Google Scholar
  • 22 Paulson S K, Kenny A D. Effect of dietary mineral and vitamin D content and parathyroidectomy on the plasma disappearance rate of 1.25-dihydroxyvitamin D3 in rats.  Biopharm Drug Dispos. 1985;  6 359-372
    PubMedSearch in Google Scholar
  • 23 Peng T C, Garner S C. Hypercalcitoninemia associated with return of serum calcium concentration toward normal in chronic parathyroidectomized rats.  Endocrinology. 1979;  104 1624-1630
    PubMedSearch in Google Scholar
  • 24 Proye C, Carnaille B, Maynou C, Bizard J P, Gilliot P, Lariviere J, Martinot J C, Monnin C, Vallier F. The parathyroid risk in thyroid surgery. Argument against early postoperative prescription of Vitamin D. Experience with 729 thyroidectomies in 1988.  Chirurgie. 1990;  116 493
    PubMedSearch in Google Scholar
  • 25 Ronga G, Fragasso G, Fiorentino A, Paserio E, Todino V, Tummarello M A. Prevalence of parathyroid insufficiency after thyroidectomy: study of 1037 cases.  Ital J Surg Sci. 1988;  18 151
    PubMedSearch in Google Scholar
  • 26 Salvesen H, Njolstad P R, Akslen L A, Albrektsen G, Viste A, Soreide O, Varhaug J E. Thyroid carcinoma: results from surgical treatment in 211 consecutive patients.  Eur J Surg. 1991;  157 493
    PubMedSearch in Google Scholar
  • 27 Sammon P J, Stacey R E, Bronner F. Role of parathyroid hormone in calcium homeostasis and metabolism.  Am J Physiol. 1970;  218 479-485
    PubMedSearch in Google Scholar
  • 28 Thomas M L, Forte L R. Sex differences during the development of vitamin D deficiency in the rat: serum parathyroid hormone, calcitonin, calcium, and phosphorus.  Endocrinology. 1981;  109 1528-1532
    PubMedSearch in Google Scholar
  • 29 Thomas M L, Armbrecht H J, Forte L R. Effects of long-term vitamin D deficiency and response to vitamin d repletion in the mature and aging male and female rat.  Mech Ageing Dev. 1984;  25 161-175
    CrossrefPubMedSearch in Google Scholar
  • 30 Torres A, Rodriguez M, Felsenfeld A, Martin-Malo A, Llach F. Sigmoidal relationship between calcitonin and calcium: Studies in the normal, parathyroidectomized, and azotemic rats.  Kidney Int. 1991;  40 700-704
    PubMedSearch in Google Scholar
  • 31 Trechsel U, Eisman J A, Fischer J A, Bonjour J P, Fleisch H. Calcium-dependent, parathyroid hormone-independent regulation of 1.25-dihydroxyvitamin D.  Am J Physiol. 1980;  239 119-124
    PubMedSearch in Google Scholar
  • 32 Wada L, Daly R, Kern D, Halloran B. Kinetics of 1. 25-dihydroxyvitamin D metabolism in the aging rat.  Am J Physiol. 1992;  262 E906-E910
    PubMedSearch in Google Scholar
  • 33 Wagner P K, Gabbert H, Strupp M, Engel T, Rothmund M. Allotransplantation der Nebenschilddrüse ohne Immunsuppression. Eine tierexperimentelle Studie.  Acta Chir Austriaca. 1988;  Sonderheft 53-56
    PubMedSearch in Google Scholar
  • 34 Wax M K, Briant T D. Completion thyroidectomy in the management of well-differentiated thyroid carcinoma.  Otolaryngol Head Neck Surg. 1992;  107 63
    PubMedSearch in Google Scholar
  • 35 Weisinger J R, Favus M J, Langman C B, Bushinsky D A. Regulation of 1.25-dihydroxyvitamin D3 by calcium in the parathyroidectomized, parathyroid hormone-replete rat.  J Bone Miner Res. 1989;  4 929-935
    PubMedSearch in Google Scholar
  • 36 Witte L W, Augostini R S, Chizmar W S. The occurrence of chronic hypocalcemia following parathyroidectomy in the green frog Rana clamitans.  Gen Comp Endocrinol. 1990;  80 419-426
    CrossrefPubMedSearch in Google Scholar

Priv.-Doz. Dr. med. C. Hasse

Coloproctology
Praxisklinik

41061 Mönchengladbach

Rathenaustraße

Germany

Phone: + 4921614624619

Fax: + 492 16 14 62 46 20

Email: coloproktologie@t-online.de