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Plant Biol (Stuttg) 2004; 6(6): 689-695
DOI: 10.1055/s-2004-821341
Original Paper

Georg Thieme Verlag Stuttgart KG · New York

Blue Light Delays Commitment to Cell Division in Chlamydomonas reinhardtii

H. Oldenhof1 , V. Zachleder2 , H. van den Ende1
  • 1Laboratory of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands
  • 2Laboratory of Cell Cycles, Institute of Microbiology, Czech Academy of Sciences, Opatovický mlýn, 379 81 Třeboň, Czech Republic
Further Information

Publication History

Received: May 1, 2004

Accepted: August 13, 2004

Publication Date:
26 October 2004 (online)

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Abstract

In this study, we describe the effect of red and blue light on the timing of commitment to cell division in Chlamydomonas reinhardtii. The time point and cell size after which cells can complete their cell cycle with one division round were determined for cultures that were exposed to various red and blue light periods. We show that the commitment point of cells grown in blue light is shifted to a later time point and a larger cell size, when compared with cells grown in red light. This shift was reduced when cultures were exposed to shorter blue light periods. Furthermore, this shift occurred only when exposure to blue light started before the cells attained a particular size. We conclude that the critical cell size for cell division, which is the cell size at which commitment to cell division is attained, is dependent on spectral composition.

Key words

Blue light - cell cycle - cell volume - Chlamydomonas reinhardtii - commitment point - red light

References

  • 1 Conlon I., Raff M.. Size control in animal development.  Cell. (1999);  96 235-244
    CrossrefPubMedSearch in Google Scholar
  • 2 Craigie R. A., Cavalier-Smith T.. Cell volume and control of the Chlamydomonas cell cycle.  Journal of Cell Science. (1982);  54 173-191
    PubMedSearch in Google Scholar
  • 3 Coleman A. W.. The nuclear cell cycle in Chlamydomonas (Chlorophyceae).  Journal of Phycology. (1982);  18 192-195
    CrossrefPubMedSearch in Google Scholar
  • 4 Donnan L., John P. C. L.. Cell cycle control by timer and sizer in Chlamydomonas. .  Nature. (1983);  304 630-633
    CrossrefPubMedSearch in Google Scholar
  • 5 Francis D.. Cell size and organ development in higher plants. Francis, D., Dudits, D., and Inzé, D., eds Plant Cell Division. London; Portland Press Ltd. (1998): 187-206
    Search in Google Scholar
  • 6 Gorman D. S., Levine R. P.. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. .  Proceedings of the National Academy of Sciences of the USA. (1965);  54 1665-1669
    PubMedSearch in Google Scholar
  • 7 Gutierrez C., Ramirez-Parra E., Castellano M. M., del Pozo J. C.. G1 to S transition: more than a cell cycle engine switch.  Current Opinion in Plant Biology. (2002);  5 480-486
    CrossrefPubMedSearch in Google Scholar
  • 8 Harper J. D. I., John P. C. L.. Coordination of division events in the Chlamydomonas cell cycle.  Protoplasma. (1986);  131 118-130
    CrossrefPubMedSearch in Google Scholar
  • 9 Lien T., Knutsen G.. Synchronous growth of Chlamydomonas reinhardtii (Chlorophyceae): a review of optimal conditions.  Journal of Phycology. (1979);  15 191-200
    CrossrefPubMedSearch in Google Scholar
  • 10 Neufeld T. P., Edgar B. A.. Connections between growth and the cell cycle.  Current Opinion in Cell Biology. (1998);  10 784-790
    CrossrefPubMedSearch in Google Scholar
  • 11 Nigg E. A.. Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle.  Bioessays. (1995);  17 471-480
    CrossrefPubMedSearch in Google Scholar
  • 12 Nurse P.. Genetic control of cell size at cell division in yeast.  Nature. (1975);  256 547-551
    PubMedSearch in Google Scholar
  • 13 Mironov V., de Veylder L., van Montagu M., Inzé D.. Cyclin-dependent kinases and cell division in plants - the nexus.  Plant Cell. (1999);  11 509-529
    CrossrefPubMedSearch in Google Scholar
  • 14 Mitchison J. M., Novak B., Sveiczer A.. Size control in the cell cycle.  Cell Biology International. (1997);  21 461-463
    CrossrefPubMedSearch in Google Scholar
  • 15 Münzner P., Voigt J.. Blue light regulation of cell division in Chlamydomonas reinhardtii. .  Plant Physiology. (1992);  99 1370-1375
    PubMedSearch in Google Scholar
  • 16 Oldenhof H., Bišová K., van den Ende H., Zachleder V.. Effect of red and blue light on the timing of cyclin-dependent kinase activity and cell division in Chlamydomonas reinhardtii. .  Plant Physiology and Biochemistry. (2004);  42 341-348
    CrossrefPubMedSearch in Google Scholar
  • 17 Parker L. L., Walter S. A., Young P. G., Piwnica-Worms H.. Phosphorylation and inactivation of the mitotic inhibitor wee1 by the nim1/cdr1 kinase.  Nature. (1993);  363 736-738
    CrossrefPubMedSearch in Google Scholar
  • 18 Polymenis M., Schmidt E. V.. Coordination of cell growth with cell division.  Current Opinion in Genetics and Development. (1999);  9 76-80
    CrossrefPubMedSearch in Google Scholar
  • 19 Rupeš I.. Checking cell size in yeast.  Trends in Genetics. (2002);  9 479-485
    PubMedSearch in Google Scholar
  • 22 Seiczer A., Novak B., Mitchison J. M.. The size control of yeast revisited.  Journal of Cell Science. (1996);  109 2947-2957
    PubMedSearch in Google Scholar
  • 20 Spudich J. L., Sager R.. Regulation of the Chlamydomonas cell cycle by light and dark.  Journal of Cell Biology. (1980);  85 136-145
    CrossrefPubMedSearch in Google Scholar
  • 21 Sueoka N.. Mitotic replication of deoxyribonucleic acid in Chlamydomonas reinhardtii. .  Proceedings of the National Academy of Sciences of the USA. (1960);  46 83-91
    PubMedSearch in Google Scholar
  • 23 Zachleder V., van den Ende H.. Cell cycle events in the green alga Chlamydomonas eugametos and their control by environmental factors.  Journal of Cell Science. (1992);  102 469-474
    PubMedSearch in Google Scholar
  • 24 Umen J. G., Goodenough U.. Control of cell division by a retinoblastoma protein homolog in Chlamydomonas. .  Genes and Development. (2001);  15 1652-1661
    CrossrefPubMedSearch in Google Scholar
  • 25 Weinberg R. A.. The retinoblastoma protein and cell cycle control.  Cell. (1995);  81 323-330
    CrossrefPubMedSearch in Google Scholar
  • 26 Zetterberg A., Larsson O., Wiman K. G.. What is the restriction point?.  Current Opinion in Cell Biology. (1995);  7 835-842
    CrossrefPubMedSearch in Google Scholar

H. Oldenhof

Laboratory of Plant Physiology
Swammerdam Institute for Life Sciences
University of Amsterdam

Kruislaan 318

1098 SM Amsterdam

The Netherlands

Email: jet_oldenhof@yahoo.com

Editor: J. T. M. Elzenga