Compounds from Epilobium angustifolium Inhibit the Specific Metallopeptidases ACE, NEP and APN

Abstract

Willow herb (Epilobium angustifolium L.) extracts showed inhibitory activity against the metallopeptidases: neutral endopeptidase (NEP), angiotensin-converting enzyme (ACE), and aminopeptidase N (APN). A bioassay-guided fractionation led to the isolation of several flavonoids and phenolic acids and an ellagitannin. The dimeric macrocyclic ellagitannin oenothein B inhibited the neutral endopeptidases in a dose-dependent manner with IC₅₀ = 20 μM. Other polyphenols showed weaker activity but their synergistic activity cannot be excluded. Taking into account the role of these peptidases in prostate diseases, the results may partly support and explain the use of Epilobium extracts in folk medicine.

Key words

Epilobium angustifolium L. - oenothein B - polyphenols - neutral endopeptidase - angiotensin-converting enzyme - aminopeptidase N

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