Abstract
Prolyl endopeptidase (PEP, EC 3.4.21.26) hydrolyzes proline-containing neuropeptides,
such as vasopressin, substance P, and thyrotropin-releasing hormone (TRH), and is
suggested to participate in learning and memory processes. Ginkgo biloba leaves, upon examination for anti-amnestic constituents as new types of PEP inhibitors,
showed significant PEP inhibition. PEP activity-guided fractionation and column chromatography
of the MeOH extracts of G. biloba leaves resulted in the isolation of 6-(8′Z-pentadecenyl)salicylic acid (1) and 6-(10′Z-heptadecenyl)salicylic acid (2). The kinetic study indicated that compounds 1 and 2 are non-competitive inhibitors of prolyl endopeptidase with Ki values of 0.87 and 0.80 μM, respectively.
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Young-Sook Paik
College of Environment and Applied Chemistry
Kyung Hee University
Suwon, 449-701
South Korea
Fax: +82-31-202-7337
Email: paikys@khu.ac.kr
