Abstract
One new diterpene, 8(14),15-sandaracopimaradiene-2α,3β,18-triol (1 ), two new phenylpropane derivatives, i. e., (E )-methyl 2-(3,4-methylene-dioxyphenyl)-3-methoxypropenoate (2 ) and (E )-2-(3,4-methylene-dioxyphenyl)-3-methoxypropenoic acid (3 ), and two known diterpenes, ent-8(14),15-sandaracopimaradiene-2α,18-diol (4 ) and 8(14),15-sandaracopimaradiene-2α,18, 19-triol (5 ), were isolated from the heartwoods and barks of Amentotaxus formosana, respectively. The anti-inflammatory activity of the diterpenes 1 , 4 , and 5 was assessed in vitro by determining their inhibitory effects on the chemical mediators released from mast cells, neutrophils, macrophages, and microglial cells. Compounds 1 , 4 , and 5 showed significant concentration-dependent inhibitory effects on the release of β-glucuronidase from rat neutrophils in response to formyl-Met-Leu-Phe/cytochalasin B (fMLP/CB) with IC50 values of 5.5 ± 1.8, 8.4 ± 2.9 and 19.2 ± 3.3 μM, respectively. Compounds 1 and 5 also showed significant concentration-dependent inhibitory effects on superoxide anion generation in rat neutrophils stimulated with fMLP/CB and phorbol 12-myristate 13-acetate (PMA) with IC50 values of 12.6 ± 1.2 and 9.4 ± 1.7, and 10.7 ± 3.3 and 12.9 ± 0.9 μM, respectively.
Key words
Amentotaxus formosana
- Amentotaxaceae - sandaracopimaradienic diterpene - anti-inflammatory - chemical mediator
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Prof. Chun-Nan Lin
School of Pharmacy
Kaohsiung Medical University
Kaohsiung 807
Taiwan
Republic of China
Phone: +886-7-312-1101∼9 ext 2163.
Fax: +886-7-556-2365
Email: lincna@cc.kmu.edu.tw