A Xylogalactofucan from the Brown Seaweed Spatoglossum schröederi Stimulates the Synthesis of an Antithrombotic Heparan Sulfate from Endothelial Cells

Abstract

The brown seaweed Spatoglossum schröederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 μg) nor hemorrhagic activities (100 μg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 μg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound.

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