Growth Parameters and Resistance against Drechslera teres of Spring Barley (Hordeum vulgare L. cv. Scarlett) Grown at Elevated Ozone and Carbon Dioxide Concentrations

M. Plessl; W. Heller; H.-D. Payer; E. F. Elstner; J. Habermeyer; I. Heiser

doi:10.1055/s-2005-873002

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2005; 7(6): 694-705
DOI: 10.1055/s-2005-873002

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Growth Parameters and Resistance against Drechslera teres of Spring Barley (Hordeum vulgare L. cv. Scarlett) Grown at Elevated Ozone and Carbon Dioxide Concentrations

M. Plessl¹ , W. Heller² , H.-D. Payer³ , E. F. Elstner¹ , J. Habermeyer¹ , I. Heiser¹

¹Institute of Phytopathology, Life Science Center Weihenstephan, Technical University of Munich, Am Hochanger 2, 85350 Freising, Germany
²GSF - National Research Center for Environment and Health, Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
³GSF - National Research Center for Environment and Health, Institute of Soil Ecology, Department of Environmental Engineering, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany

Abstract

Spring barley (Hordeum vulgare L. cv. Scarlett) was grown at two CO₂ levels (400 vs. 700 ppm) combined with two ozone regimes (ambient vs. double ambient) in climate chambers for four weeks, beginning at seedling emergence. Elevated CO₂ concentration significantly increased aboveground biomass, root biomass, and tiller number, whereas double ambient ozone significantly decreased these parameters. These ozone-induced reductions in growth parameters were strongly overridden by 700 ppm CO₂. The elevated CO₂ level increased C : N ratio of the leaf tissue and leaf starch content but decreased leaf protein levels. Exposure to double ambient ozone did not affect protein content and C : N ratio but dramatically increased leaf starch levels at 700 ppm CO₂. Resistance against Drechslera teres (Sacc.) Shoemaker was increased in leaves grown at double ambient ozone but was less obvious at 700 ppm than at 400 ppm CO₂. Constitutive activities of β‐1,3-glucanase and chitinase were significantly higher in leaves grown at double ambient ozone compared to ambient ozone levels. The sum of methanol-soluble and alkali-released cell wall-bound aromatic metabolites (i.e., C-glycosylflavones and several structurally unidentified metabolites) and lignin contents did not show any treatment-dependent differences.

Key words

Net blotch disease (Drechslera teres) - Hordeum vulgare - induced resistance - PR proteins

Full Text

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I. Heiser

Institute of Phytopathology
Life Science Center Weihenstephan
Technical University of Munich

Am Hochanger 2

85350 Freising

Germany

Email: heiser@lrz.tum.de

Guest Editor: R. Matyssek