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J Reconstr Microsurg 2005; 21(7): 483-489
DOI: 10.1055/s-2005-918904
Copyright © 2005 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Role of Simple Cold Storage in Preventing Epiphyseal Growth Plate Impairment after Replantation Surgery in Immature Rats

Toru Sunagawa1 , Osamu Ishida1 , Yoshikazu Ikuta1 , Yuji Yasunaga1 , Mitsuo Ochi1
  • 1Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan
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Publication History

Accepted: June 6, 2005

Publication Date:
30 September 2005 (online)

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ABSTRACT

The purpose of this study was to investigate the effect of ischemia time on longitudinal bone growth after replantation, and to evaluate the usefulness of simple cold storage of an amputated limb in preventing epiphyseal growth-plate impairment. A hind-limb replantation model was produced with 5-week-old rats after various ischemia times.

With more than 6 hr of warm ischemia, growth disturbance was observed, and extensive necrosis was histologically apparent in the central region of the growth plate of the proximal tibia, even at 1 week postoperatively. Destruction of the growth plate was complete at 4 weeks after surgery. By simple cold storage of the amputated limb, growth disturbance was not observed, even following ischemia for 9 hr, and no abnormal findings were observed histologically.

Segmental necrosis and destruction of the growth plate observed histologically suggested the possibility of indirect impairment of the epipyseal chondrocytes due to disturbance of the epiphyseal arterial system, attributable to ischemia. The study confirmed experimentally that cold storage of the amputated part may prevent longitudinal bone-growth disturbance after extension of ischemia time.

KEYWORDS

Replantation surgery - ischemia - bone growth - growth plate - cold storage - rat model

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Toru Sunagawa

Dept. of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University

1-2-3 Kasumi, Hiroshima, 734-8551, Japan