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Plant Biol (Stuttg) 2006; 8(4): 450-461
DOI: 10.1055/s-2006-923951
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Development of NaCl-Tolerant Strain in Chrysanthemum morifolium Ramat. through in vitro Mutagenesis

Z. Hossain1 , 3 , A. K. A. Mandal1 , S. K. Datta1 , A. K. Biswas2
  • 1Botanic Garden and Floriculture Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India
  • 2Cytogenetics and Plant Breeding Laboratory, Botany Department, University of Kalyani, Kalyani - 741235, Nadia, West Bengal, India
  • 3Present address: Botany Department, Rishi Bankim Chandra College, Naihati, 24-Parganas (N)-743165, West Bengal, India
Further Information

Publication History

Received: August 27, 2005

Accepted: January 17, 2006

Publication Date:
11 May 2006 (online)

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Abstract

One NaCl-tolerant chrysanthemum (Chrysanthemum morifolium Ramat.) variant (E2) has been developed in a stable form through in vitro mutagenesis using ethylmethane sulfonate (EMS) as the chemical mutagen. Salt tolerance was evaluated by the capacity of the plant to maintain both flower quality and yield under stress conditions. Enhanced tolerance of the E2 variant has been attributed to the increased activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), and dehydroascorbate reductase (DHAR), and, to a lesser extent of membrane damage than NaCl-treated control plants. Isoform analysis revealed that an increase in total SOD activity in the E2 variant was solely due to significant activation of the Cu/Zn isoform. Elevated levels of carotenoids and ascorbate in E2 leaves have been reflected in their higher free radical scavenging capacity (RSC) expressed in terms of DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging ability. Data reflect that a proper balance between enzymatic and non-enzymatic defence systems is required for combating salinity stress in chrysanthemum. Better performance of the E2 progeny under same salinity stress condition, even in the second year, confirms the genetic stability of the salt-tolerance character. On the whole, the E2 variant, developed through 0.025 % EMS treatment, might be considered as a NaCl-tolerant strain showing positive characters towards NaCl stress.

Key words

Antioxidant enzymes - salinity stress - reactive oxygen species - NaCl-tolerant - in vitro mutagenesis - EMS - chrysanthemum

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S. K. Datta

Botanic Garden and Floriculture Division
National Botanical Research Institute

Rana Pratap Marg

Lucknow - 226001, Uttar Pradesh

India

Email: subodhdatta@usa.net

Editor: J. T. M. Elzenga

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