Abscisic Acid Stimulates the Expression of Two Isoforms of Plasma Membrane Ca²⁺-ATPase in Arabidopsis thaliana Seedlings

Abstract

At-ACA8 and At-ACA9 are two plasma membrane (PM) Ca²⁺-ATPases of Arabidopsis thaliana. In this article the expression of At-ACA8, At-ACA9, and of At-ACA10, a third isoform of Ca²⁺-ATPase closely related to PM Ca²⁺-ATPases, was analysed and the effect of the hormone abscisic acid (ABA) on the expression level of PM Ca²⁺-ATPase specific transcripts was investigated. In adult plants of A. thaliana, At-ACA8 and At-ACA10 are expressed in all organs considered whereas At-ACA9 is expressed only in flowers. All isoforms of PM Ca²⁺-ATPases can be detected in young seedlings but the amount of At-ACA9 mRNA is much lower than those of At-ACA8 and At-ACA10. ABA markedly and rapidly stimulates the expression of both At-ACA8 and At-ACA9 genes in young seedlings but not that of At-ACA10. ABA also increases the level of At-ACA8 protein at the PM, suggesting a role for PM Ca²⁺-ATPases in ABA signalling.

Gene expression - plant type II B Ca²⁺-ATPases - abscisic acid - RT‐PCR - real-time PCR - plasma membrane

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Editor: J. Schroeder