A Novel Rice MAPK Gene, OsBIMK2, is Involved in Disease-Resistance Responses

D. Song; J. Chen; F. Song; Z. Zheng

doi:10.1055/s-2006-924149

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2006; 8(5): 587-596
DOI: 10.1055/s-2006-924149

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

A Novel Rice MAPK Gene, OsBIMK2, is Involved in Disease-Resistance Responses

D. Song¹ ^, ² ^, ⁴ , J. Chen¹ ^, ² , F. Song¹ ^, ² ^, ³ , Z. Zheng¹ ^, ²

¹Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
²Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
³State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, Zhejiang 310029, P.R. China
⁴Present address: Research Center for Cyanobacterial and Algal Biotechnology, College of Marine Science and Engineering, Tianjin University of Science and Technology, TEDA, Tianjin 300457, P.R. China

Abstract

The mitogen-activated protein kinase (MAPK) cascades play important roles in transmission of extracellular signals to the downstream effector proteins through a mechanism of protein phosphorylation. In this study, we isolated and identified a novel rice MAPK gene, OsBIMK2 (Oryzae sativa L. BTH-Induced MAP Kinase 2). The OsBIMK2 encodes a 506 amino acid protein with molecular weight of 63 kD. The recombinant OsBIMK2 expressed in Escherichia coli showed an autophosphorylation activity in vitro. OsBIMK2 is a single-copy gene in the rice genome. Expression of OsBIMK2 was activated upon treatment with benzothiadiazole (BTH), which is capable of inducing disease resistance in rice. Expression of OsBIMK2 was also up-regulated during early stage after inoculation with Magnaporthe grisea in BTH-treated rice seedlings and during an incompatible interaction between M. grisea and a blast-resistant rice genotype. Over-expression of the rice OsBIMK2 gene in transgenic tobacco resulted in an enhanced disease resistance against tomato mosaic virus and a fungal pathogen, Alternaria alternata. These results suggest that OsBIMK2 plays a role in disease resistance responses.

Key words

MAPK cascades - benzothiadiazole (BTH) - Magnaporthe grisea - disease resistance response - rice (Oryza sativa L.) - transgenic tobacco - tomato mosaic virus (ToMV) - Alternaria alternata

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F. Song

Department of Plant Protection
College of Agriculture and Biotechnology
Zhejiang University

Hangzhou

Zhejiang 310029

P.R. China

Email: fmsong@zju.edu.cn

Editor: J. Cullimore