Nectar Resorption and Translocation in Cucurbita pepo L. and Platanthera chlorantha Custer (Rchb.)

M. Nepi; M. Stpiczyńska

doi:10.1055/s-2006-924287

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Plant Biol (Stuttg) 2007; 9(1): 93-100
DOI: 10.1055/s-2006-924287

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Nectar Resorption and Translocation in Cucurbita pepo L. and Platanthera chlorantha Custer (Rchb.)

M. Nepi¹ , M. Stpiczyńska²

¹Department of Environmental Sciences “G. Sarfatti”, Botany Section, Siena University, via PA Mattioli 4, 53100 Siena, Italy
²Department of Botany, Lublin Agricultural University, ul. Akademicka 15, 20-950 Lublin, Poland

Further Information

Publication History

Received: March 2, 2006

Accepted: May 11, 2006

Publication Date:
01 August 2006 (online)

Abstract
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Abstract

Nectar resorption and sugar translocation were studied in Cucurbita pepo (Cucurbitaceae) and Platanthera chlorantha (Orchidaceae) by micro-autoradiography. In both species, nectar was resorbed in pollinated and unpollinated flowers and ovules developing into seeds were found to be the main sugar sink. In C. pepo, the mobility of resorbed sugars in pollinated female flowers was higher than in unpollinated ones; male flowers showed lower mobility of resorbed sugar. In P. chlorantha, radioactivity was detected in pollinated flowers below and above labelled unpollinated ones: the nearer the flower, the stronger the accumulation of label in developing fruits.

Key words

Cucurbita pepo - Platanthera chlorantha - floral nectary - pollination - nectar resorption - micro-autoradiography.

References

1 Akazawa T., Okamoto K.. Biosynthesis and metabolism of sucrose. Stumpf, P. K. and Conn, E. E., eds. The Biochemistry of Plants, a Comprehensive Treatise. New York; Academic Press (1980): 199-220

Search in Google Scholar
2 Andronova E. V.. Embriogienez orchidnych. Batygina, T. B., ed. Embriologia Cvietkovych Rastienii II. Sankt Petersburg; Mir i Siemja (1998): 545-556

Search in Google Scholar
3 Ashworth L., Galetto L.. Differential nectar production between male and female flowers in wild cucurbit: Cucurbita maxima ssp. andreana (Cucurbitaceae). Canadian Journal of Botany. (2002); 80 1203-1208

Crossref PubMed Search in Google Scholar
4 Baker H. G., Baker I.. Chemical constituents of nectar in relation to pollination mechanisms and phylogeny. Nitecki, M. H., ed. Biochemical Aspects of Evolutionary Biology. Chicago; University of Chicago Press (1983): 131-171

Search in Google Scholar
5 Bednara J., Kuraś M., Rodkiewicz B.. Ultrastructural changes during megasporogenesis in Epipactis (Orchidaceae). Acta Societatis Botanicorum Poloniae. (1981); 50 127-130

PubMed Search in Google Scholar
6 van Bel A. J. E.. The phloem, a miracle of ingenuity. Plant, Cell and Environment. (2003); 26 125-149

Crossref PubMed Search in Google Scholar
7 Bernardello L., Galetto L., Rodriguez I. G.. Reproductive biology, variability of nectar features and pollination of Combretum fruticosum (Combretaceae) in Argentina. Botanical Journal of the Linnean Society. (1994); 114 293-308

Crossref PubMed Search in Google Scholar
8 Bieleski R. L., Redgwell R. J.. Sorbitol metabolism in nectaries from flowers of Rosaceae. Australian Journal of Plant Physiology. (1980); 7 15-25

PubMed Search in Google Scholar
9 Bonnier G.. Les nectaries. American Scientific Naturalist. (1878); 8 5-212

PubMed Search in Google Scholar
10 Búrquez A., Corbet S. A.. Do flowers resorb nectar?. Functional Ecology. (1991); 5 369-379

Crossref PubMed Search in Google Scholar
11 Cruden R. W., Hermann S. M., Peterson S.. Patterns of nectar production and plant-pollinator coevolution. Bentley, B. and Elias, T. S., eds. The Biology of Nectaries. New York; Columbia University Press (1983): 80-125

Search in Google Scholar
12 Davis A. R.. Influence of floral visitation on nectar-sugar composition and nectary surface changes in Eucalyptus. Apidologie. (1997); 28 27-42

PubMed Search in Google Scholar
13 Fahn A., Rachmilewitz T.. An autoradiographical study of nectar secretion in Lonicera japonica Thumb. Annals of Botany. (1975); 39 975-976

PubMed Search in Google Scholar
14 Giaquinta R. T.. Translocation of sucrose and oligosaccharides. Stumpf, P. K. and Conn, E. E., eds. The Biochemistry of Plants, a Comprehensive Treatise. New York; Academic Press (1980): 271-320

Search in Google Scholar
15 Heinrich G.. Über den Glucose-Metabolismus in Nektarien zweier Aloe-Arten und über den Mechanismus der Pronektar-Sekretion. Protoplasma. (1975); 85 351-371

Crossref PubMed Search in Google Scholar
16 Heinrich G., Welz W., Sawidis T.. Substanzverluste und Strukturerhaltung im Verlauf einer „trockenen“ Einbettung für die Elektronenmikroskopie. Phyton. (1987); 27 299-309

PubMed Search in Google Scholar
17 Koopowitz H., Marchant T. A.. Postpollination nectar resorption in the African epiphyte Aerangis verdickii (Orchidaceae). American Journal of Botany. (1998); 85 508-512

Crossref PubMed Search in Google Scholar
18 Langenberger M. W., Davis A. R.. Temporal changes in floral nectar production, reabsorption, and composition associated with dichogamy in annual caraway (Carum carvi, Apiaceae). American Journal of Botany. (2002); 89 1588-1598

PubMed Search in Google Scholar
19 Lalonde S., Tegeder M., Throne-Holst M., Frommer W. B., Patrick J. W.. Phloem loading and unloading of sugars and amino acids. Plant, Cell and Environment. (2003); 26 37-56

Crossref PubMed Search in Google Scholar
20 Luyt R., Johnson S. D.. Postpollination nectar resorption and its implications for fruit quality in an epiphytic orchid. Biotropica. (2002); 34 442-446

PubMed Search in Google Scholar
21 Nepi M., Ciampolini F., Pacini E.. Development and ultrastructure of Cucurbita pepo nectaries of male flowers. Annals of Botany. (1996 a); 78 95-104

Crossref PubMed Search in Google Scholar
22 Nepi M., Guarnieri M., Pacini E.. Nectar secretion, resorption, and sugar composition in male and female flowers of Cucurbita pepo. International Journal of Plant Sciences. (2001); 162 353-358

Crossref PubMed Search in Google Scholar
23 Nepi M., Pacini E.. Effect of pistil age on pollen tube growth, fruit development and seed set in Cucurbita pepo L. Acta Societatis Botanicorum Poloniae. (2001); 70 165-172

PubMed Search in Google Scholar
24 Nepi M., Pacini E., Willemse M. T. M.. Nectary biology of Cucurbita pepo: ecophysiological aspects. Acta Botanica Neerlandica. (1996 b); 45 41-54

PubMed Search in Google Scholar
25 Nicolson S. W.. Direct demonstration of nectar resorption in the flowers of Grevillea robusta (Proteaceae). Functional Ecology. (1995); 9 584-588

Crossref PubMed Search in Google Scholar
26 Pedersen M. W., LeFevre C. W., Wiebe H. H.. Absorption of C¹⁴-labelled sucrose by alfalfa nectaries. Science (Washington, D.C.). (1958); 127 758-759

PubMed Search in Google Scholar
27 Peng Y.-B., Li Y.-Q., Hao Y.-J., Xu Z.-H., Bai S.-N.. Nectar production and transportation in the nectaries of the female Cucumis sativus L. flower during anthesis. Protoplasma. (2004); 224 71-78

PubMed Search in Google Scholar
28 Pyke G. H.. What does it cost a plant to produce nectar?. Nature. (1991); 350 58-59

PubMed Search in Google Scholar
29 Reynolds E. S.. The use of lead citrate at pH as an eloctron-opaque stain in electron microscopy. Journal of Cell Biology. (1963); 17 208-212

Crossref PubMed Search in Google Scholar
30 Riveira G. L., Galetto L., Bernardello L.. Nectar secretion pattern, removal effect, and breeding system of Ligaria cuneifolia (Loranthaceae). Canadian Journal of Botany. (1996); 74 1996-2001

PubMed Search in Google Scholar
31 Sawidis T., Heinrich G., Tsekos I.. Autoradiographical study of the incorporation of tritium labelled glucose (D-glucose-6-H³) in floral nectaries of Abutilon striatus (Dicks). Bios (Thessaloniki). (1989); 1 211-219

PubMed Search in Google Scholar
32 Shuel R. W.. Influence of reproductive organs on secretion of sugars in flowers of Streptosolen jamesonii Miers. Plant Physiology. (1961); 36 265-271

PubMed Search in Google Scholar
33 Southwick E. E.. Photosynthate allocation to floral nectar: a neglected energy investment. Ecology. (1984); 65 1775-1779

Crossref PubMed Search in Google Scholar
34 Stephenson A. G., Winsor J. A., Schlichting C. D.. Evidence for non-random fertilisation in the common zucchini, Cucurbita pepo. Cresti, M., Gori, P., and Pacini E., eds. Sexual Reproduction in Higher Plants. Berlin; Springer Verlag (1988): 333-338

Search in Google Scholar
35 Stpiczyńska M.. Nectar resorption in the spur of Platanthera chlorantha Custer (Rchb.) Orchidaceae - structural and microautoradiographic study. Plant Systematics and Evolution. (2003 a); 238 119-126

PubMed Search in Google Scholar
36 Stpiczyńska M.. Incorporation of [³H]sucrose after the resorption of nectar from the spur of Platanthera chlorantha (Custer) Rchb. Canadian Journal of Botany. (2003 b); 81 927-932

Crossref PubMed Search in Google Scholar
37 Stpiczyńska M., Pielecki J.. Sekrecja, resorbcja i skład chemiczny nektaru podkolana zielonawego Platanthera chloantha (Custer) Rchb. (Orchidaceae). Annales UMCS Sectio EEE. (2002); 10 173-179

PubMed Search in Google Scholar
38 Stpiczyńska M.. Rola nektaru w kwiatach podkolanu zielonawego Platanthera chloantha (Custer) Rchb. (Orchidaceae). Rozprawy Naukowe Akademii Rolniczej w Lublinie, WAR. (2004); 286 1-103

PubMed Search in Google Scholar
39 Stpiczyńska M., Milanesi C., Faleri C., Cresti M.. Ultrastructure of the nectary spur of Platanthera chlorantha (Custer) Rchb. (Orchidaceae) during successive stages of nectar secretion. Acta Biologica Cracoviensia Series Botanica. (2005); 47 111-119

PubMed Search in Google Scholar
40 Tissue D. T., Skillman J. B., McDonald E. P., Strain B. R.. Photosynthesis and carbon allocation in Tipularia discolor (Orchidaceae), a wintergreen understory herb. American Journal of Botany. (1995); 82 1249-1256

Crossref PubMed Search in Google Scholar
41 Torres C., Galetto L.. Patterns and implications of floral nectar secretion, chemical composition, removal effects and standing crop in Mandevilla pentlandiana Apocynaceae. Botanical Journal of the Linnean Society. (1998); 127 207-223

Crossref PubMed Search in Google Scholar
42 Van Die J., Leeuwangh P., Hoekstra S. M. R.. Translocation of assimilates in Fritillaria imperialis L. 1. The secretion of ¹⁴C-labelled sugars by the nectaries in relation to phylotaxis. Acta Botanica Neerlandica. (1970); 19 16-23

PubMed Search in Google Scholar
43 Veyret Y.. Development of the embryo and the young seedling stages of orchids. Withner, C. L., ed. The Orchids : Scientific Studies. New York; Wiley and Sons (1974): 223-265

Search in Google Scholar
44 Wittich P. E., Willemse M. T. M.. Sucrose utilization during ovule and seed development of Gasteria verrucosa (Mill.) H. Duval as monitored by sucrose synthase and invertase localization. Protoplasma. (1999); 208 136-148

Crossref PubMed Search in Google Scholar
45 Zauralov O. A., Pavlinova O. A.. Transport and conversion of sugars in nectaries in connection with the secretory function. Fiziologiya Rastenii. (1975); 22 500-507

PubMed Search in Google Scholar
46 Zauralov O. A., Yakovleva L. P.. Sostav sacharov nektara niekotorych miedonosnych rastenii. Rastitelnye Resursy. (1973); 9 444-451

PubMed Search in Google Scholar

M. Nepi

Department of Environmental Sciences “G. Sarfatti”
Botany Section
Siena University

via PA Mattioli 4

53100 Siena

Italy

Email: nepim@unisi.it

Editor: A. Weber