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Plant Biol (Stuttg) 2007; 9(1): 85-92
DOI: 10.1055/s-2006-924347
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Structural-Functional Changes in Detached Cucumber Leaves, and Modelling These by Hormone-Treated Leaf Discs

E. Kovács1 , É. Sárvári2 , P. Nyitrai2 , J. Darók3 , E. Cseh2 , F. Láng2 , Á. Keresztes1
  • 1Department of Plant Anatomy, Eötvös L. University, Pázmány P. sétány 1/C, 1117 Budapest, Hungary
  • 2Department of Plant Physiology and Molecular Plant Biology, Eötvös L. University, Pázmány P. sétány 1/C, 1117 Budapest, Hungary
  • 3Department of Plant Taxonomy and Geobotany, University of Pécs, Ifjuság útja 6, 7624 Pécs, Hungary
Further Information

Publication History

Received: March 12, 2006

Accepted: May 22, 2006

Publication Date:
28 September 2006 (online)

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Abstract

Senescence and rejuvenation were investigated in detached cucumber (Cucumis sativus L.) leaves after cultivation in nutrient solution for one week or four weeks. Rooting of the petiole (visible generally from the 7th day) elicited a combination of different morphological, anatomical, and physiological changes in the lamina. Extensive growth in area and thickness, extreme regreening, changes of chloroplast structure and activity, as well as the pattern of Chl-protein complexes were observed and compared either to the corresponding parameters of young detached leaves or mature attached leaves. These responses could be provoked separately by treating excised leaf discs with kinetin, benzyladenine, or indolylacetic acid. The hormones showed mutuality in their effects, benzyladenine being responsible for the growth of cells, while indolylacetic acid and kinetin promoted an increase in chlorophyll content. However, none of the treatments resulted in the growth of the chloroplasts in the leaf discs, which was only prominent in the rooting leaves.

Key words

Chlorophyll - chloroplast - Cucumis sativus L. - cytokinins - photosynthesis - rejuvenation - senescence

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E. Kovács

Department of Plant Anatomy
Eötvös L. University

Pázmány P. sétány 1/C

1117 Budapest

Hungary

Email: k-erika@elte.hu

Editor: J. T. M. Elzenga