Abstract
Phellinsin A, which was isolated from the culture broth of Phellinus sp. PL3, exhibited significant low-density lipoproteins (LDL)-antioxidant activity. It inhibited the Cu2+-mediated oxidation of LDL (IC50: 5.3 μM) and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-mediated oxidation of LDL (IC50: 2.8 μM) in the thiobarbituric acid-reactive substances (TBARS) assay as well as the macrophage-mediated LDL oxidation (73 % inhibition at 5 μM). In addition, it delayed LDL oxidation with a prolonged lag time (192 min at 2 μM, control: 44 min). This compound also showed a 10-fold more potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50: 1.7 μM) than trolox (IC50: 18.6 μM), a known DPPH inhibitor. In addition, phellinsin A inhibited xanthine oxidase activity with an IC50 value of 31.0 μM, whereas allopurinol, a xanthine oxidase inhibitor, showed an IC50 value of 40.7 μM.
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Dr. Sung Uk Kim
Division of Drug Discovery
Korea Research Institute of Bioscience and Biotechnology
Yusung
Daejeon 305-333
Korea
Phone: +82-42-860-4554
Fax: +82-42-861-2675
Email: kimsu@kribb.re.kr
