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Planta Med 2006; 72(6): 572-575
DOI: 10.1055/s-2006-931533
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Phellinsin A from Phellinus sp. PL3 Exhibits Antioxidant Activities

Eui Il Hwang1 , 3 , Ju-Ryoung Kim2 , 3 , Tae-Sook Jeong2 , Sangku Lee2 , Mun-Chual Rho2 , Sung Uk Kim2
  • 1Agro-Tech Research Group, KT&G Central Research Institute, Daejeon 305-805, Korea
  • 2Division of Drug Discovery, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea
  • 3These authors contributed equally to this work
Further Information

Publication History

Received: August 28, 2005

Accepted: December 6, 2005

Publication Date:
24 April 2006 (online)

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Abstract

Phellinsin A, which was isolated from the culture broth of Phellinus sp. PL3, exhibited significant low-density lipoproteins (LDL)-antioxidant activity. It inhibited the Cu2+-mediated oxidation of LDL (IC50: 5.3 μM) and 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH)-mediated oxidation of LDL (IC50: 2.8 μM) in the thiobarbituric acid-reactive substances (TBARS) assay as well as the macrophage-mediated LDL oxidation (73 % inhibition at 5 μM). In addition, it delayed LDL oxidation with a prolonged lag time (192 min at 2 μM, control: 44 min). This compound also showed a 10-fold more potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity (IC50: 1.7 μM) than trolox (IC50: 18.6 μM), a known DPPH inhibitor. In addition, phellinsin A inhibited xanthine oxidase activity with an IC50 value of 31.0 μM, whereas allopurinol, a xanthine oxidase inhibitor, showed an IC50 value of 40.7 μM.

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Dr. Sung Uk Kim

Division of Drug Discovery

Korea Research Institute of Bioscience and Biotechnology

Yusung

Daejeon 305-333

Korea

Phone: +82-42-860-4554

Fax: +82-42-861-2675

Email: kimsu@kribb.re.kr