Planta Med 2006; 72(8): 715-720
DOI: 10.1055/s-2006-941507
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Anti-arthritic activity of a lipophilic woad (Isatis tinctoria) extract

María-Carmen Recio1 , Miguel Cerdá-Nicolás2 , Matthias Hamburger3 , José-Luis Ríos1
  • 1Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Spain
  • 2Department of Pathology, Faculty of Medicine, University of Valencia, Spain
  • 3Institute of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Switzerland
Further Information

Publication History

Received: 23.12.2005

Accepted: 20.4.2006

Publication Date:
31 May 2006 (online)

Abstract

A dichloromethane extract of Isatis tinctoria was tested in the adjuvant-induced arthritis model in rats. The extract (150 mg/kg p. o.) leads to a significant reduction of paw oedema. Radiographic, histological and clinical assessment confirmed reduced damage of cartilage and signs of inflammatory response in comparison to untreated control. No significant differences were observed in the tissular levels of cyclooxygenases1 and -2, and of inducible nitric oxide synthase in Isatis treated and untreated animals. High dose treatment with Isatis extract for two weeks did not result in macroscopic lesions of the gastric mucosa.

References

  • 1 Shmerling R H. Rheumatoid arthritis.  Drugs Today (Barc). 1998;  34 649-53
  • 2 Smolen J S, Steiner G. Therapeutic strategies for rheumatoid arthritis.  Nat Rev Drug Discov. 2003;  2 473-88
  • 3 Lundberg I E, Grundtman C, Larsson E, Klareskog L. Corticosteroids - from an idea to clinical use.  Best Pract Res Clin Rheumatol. 2004;  18 7-19
  • 4 Taurog J D, Argentieri D C, McReynolds R A. Adjuvant arthritis.  Methods Enzymol. 1988;  162 339-55
  • 5 Cuzzocrea S, Mazzon E, Bevilaqua C, Costantino G, Britti D, Mazullo G. et al . Cloricromene, a coumarine derivative, protects against collagen-induced arthritis in Lewis rats.  Br J Pharmacol. 2000;  131 1399-407
  • 6 Hamburger M. Isatis tinctoria - from the rediscovery of an ancient medicinal plant towards a novel anti-inflammatory phytopharmaceutical.  Phytochem Rev. 2002;  1 333-44
  • 7 Danz H, Stoyanova S, Wippich P, Brattström A, Hamburger M. Identification and isolation of the cyclooxygenase-2 inhibitory principle in Isatis tinctoria .  Planta Med. 2001;  67 411-6
  • 8 Danz H, Stoyanova S, Thomet O AM, Simon H U, Dannhardt G, Ulbrich H. et al . Inhibitory activity of tryptanthrin on prostaglandin and leukotriene synthesis.  Planta Med. 2002;  68 875-80
  • 9 Ishihara T, Kohno K, Ushio S, Ikeda M, Kurimoto M. Tryptanthrin inhibits nitric oxide and prostaglandin E2 synthesis by murine macrophages.  Eur J Pharmacol. 2000;  407 197-204
  • 10 Rüster G -U, Hoffmann B, Hamburger M. Inhibitory activity of indolin-2-one derivatives on compound 48/80-induced histamine release from mast cells.  Pharmazie. 2004;  59 236-7
  • 11 Oberthür C, Jäggi U, Hamburger M. HPLC-based activity profiling of a lipophilic Isatis tinctoria leaf extract for 5-lipoxygenase inhibitory activity.  Fitoterapia. 2005;  76 324-32
  • 12 Recio M C, Cerdá-Nicolás M, Potterat O, Hamburger M, Ríos J L. Anti-inflammatory and anti-allergic activity in vivo of lipophilic Isatis tinctoria extracts and tryptanthrin.  Planta Med. 2006;  72 539-46
  • 13 Heinemann C, Schliemann-Willers S, Oberthür C, Hamburger M, Elsner P. Prevention of experimentally induced irritant contact dermatitis by extracts of Isatis tinctoria compared to pure tryptanthrin and its impact on UVB-induced erythema.  Planta Med. 2004;  70 385-90
  • 14 De León E J, Alcaraz M J, Domínguez J N, Charris J, Terencio M C. 1-(2,3,4-trimethoxyphenyl)-3-(3(2-chloroquinolinyl))-2-propen-1-one, a chalcone derivative with analgesic, anti-inflammatory and immunomodulatory properties.  Inflamm Res. 2003;  52 246-57
  • 15 Llombart-Bosch A, Navarro S. Immunohistochemical detection of EWS and FLI-1 proteins in Ewing sarcoma and primitive neuroectodermal tumors: comparative analysis with CD99 (MIC-2) expression.  Appl Immunohistochem Mol Morphol. 2001;  9 255-60
  • 16 Ghosh S, May M J, Kopp E B. NF-κB and rel proteins: Evolutionarily conserved mediators of immune responses.  Annu Rev Immunol. 1998;  16 225-60
  • 17 Tak P T, Firestein G S. NF-κB: a key role in inflammatory diseases.  J Clin Invest. 2001;  107 7-11
  • 18 Bremner P, Heinrich M. Natural products as targeted modulators of the nucleat factor-κB pathway.  J Pharm Pharmacol. 2002;  54 453-72

Prof. José-Luis Ríos

Department of Pharmacology

Faculty of Pharmacy

University of Valencia

Av. Vicent Andrés Estellés

s/n. 46100 Burjassot

Valencia

Spain

Phone: +34963544973

Fax: +34963544973

Email: riosjl@uv.es