An Approach to the Inheritance of the Sesquiterpene Chemotypes within Petasites hybridus

Abstract

Sesquiterpene esters are the active principle in the medicinal plant Petasites hybridus. Two chemotypes, the petasin chemotype and the furanopetasin chemotype, are known, but only the first one is suitable for pharmaceutical purposes. Experimental crossings were performed within and between plants of both chemotypes to study the genetic basis of the occurrence of these sesquiterpenes. The chemotype was determined by TLC in extracts of a small piece of rhizome in the parent plants and the progenies. A model including the combined action of two genes is proposed to explain the inheritance of the chemotypes where the furanopetasin chemotype is under recessive genetic control.

References

• 1 Meier B, Meier-Liebi M. Petasites. In: Hänsel R, Keller K, Rimpler H, Schneider G, editors Hagers Handbuch der Pharmazeutischen Praxis. 5th edition, Vol 6 Berlin Heidelberg; Springer Verlag 1994: p 81-105
• 2 Barrett M. The handbook of clinically tested herbal remedies. Binghampton; Haworth Herbal Press 2004: p 213-23
• 3 Wildi E, Langer T, Schaffner W, Berger Büter K. Quantitative analysis of petasin and pyrrolizidine alkaloids in leaves and rhizomes of in situ grown Petasites hybridus plants.  Planta Med. 1998;  64 264-7
• 4 Novotny L, Toman J, Stary F, Marquez A D, Herout V, Sorm F. Contribution to the chemotaxonomy of some European Petasites species.  Phytochemistry. 1966;  5 1281-7
• 5 Debrunner B, Neuenschwander M, Brenneisen R. Sesquiterpenes of Petasites hybridus (L.) G.M. et Sch.: distribution of sesquiterpenes over plant organs.  Pharm Acta Helv. 1995;  70 167-73
• 6 Debrunner B, Neuenschwander M. Sesquiterpenes of Petasites hybridus (L.) G.M. et Sch.: influence of locations and seasons on sesquiterpene distribution.  Pharm Acta Helv. 1995;  70 315-23
• 7 Siegenthaler P. Untersuchungen zur Struktur und Analytik der Inhaltsstoffe von Petasites albus und Petasites hybridus (Furanopetasin-Chemovarietät) [dissertation]. Bern; University of Bern 1995
• 8 Chizzola R, Ozlsberger B, Langer T. Variability in chemical constituents in Petasites hybridus from Austria.  Biochem Syst Ecol. 2000;  28 421-32
• 9 Vetter S, Franz C, Glasl S, Kastner U, Saukel J, Jurenitsch J. Inheritance of sesquiterpene lactone types within the Achillea millefolium complex (Compositae).  Plant Breed. 1997;  116 79-82
• 10 Franz C, Wickel J. Zur Vererbung der Bestandteile des Kamillenöls. 1. Mitt.: Qualitative Vererbung von Chamazulen und (-)-α-Bisabolol.  Herba Hung. 1985;  24 49-59
• 11 Horn W, Franz C, Wickel I. Genetics of bisaboloids in chamomile.  Plant Breed. 1988;  101 307-12
• 12 Thompson J D, Chalchat J C, Michet A, Linhart Y B, Ehlers B. Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes.  J Chem Ecol. 2003;  29 859-80
• 13 Chizzola R, Langer T. Distribution of pyrrolizidine alkaloids in crossing progenies of Petasites hybridus .  J Herbs Spices Med Plants. 2002;  9 39-44; As book chapter in: Johnson CB, Franz C, editors. Breeding research on aromatic and medicinal plants. New York: Haworth Press; 2002. p 39 - 44
• 14 Debrunner B, Meier B. Petasites hybridus: A tool for interdisciplinary research in phytotherapy.  Pharm Acta Helv. 1998;  72 359-80
• 15 Wagner H, Bladt S. Plant drug analysis. A thin layer chromatography atlas. 2nd edition Berlin; Springer 1996: p 359