Abstract
During batch cultivation, the cyanobacterial strain Synechocystis aquatilis Sauvageau B90.79 was found to release a fucose-rich sulfated polysaccharide, which was mainly composed of arabinose (45 %) and fucose (47 %) and had a degree of sulfation of 0.43. The released polysaccharide showed merely an anticoagulant activity of less than 10 % compared to the reference compound, unfractionated heparin. However, distinctive effects on the complement activation were observed: its inhibitory effect on the classical pathway of complement activation was 600-fold stronger than that of unfractionated heparin, whereas that on the alternative pathway of complement activation was 2- to 3-fold weaker. The results indicate that this biotechnologically producible, released polysaccharide represents a specifically acting complement modulator.
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Dr. Rainer-B. Volk
Pharmaceutical Institute
University of Kiel
Gutenbergstraße 76
24118 Kiel
Germany
Telefon: +49-431-880-1144
Fax: +49-431-880-1102
eMail: volk@pharmazie.uni-kiel.de
