Truncated Hemoglobins in Actinorhizal Nodules of Datisca glomerata

K. Pawlowski; K. R. Jacobsen; N. Alloisio; R. Ford Denison; M. Klein; J. D. Tjepkema; T. Winzer; A. Sirrenberg; C. Guan; A. M. Berry

doi:10.1055/s-2007-965258

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2007; 9(6): 776-785
DOI: 10.1055/s-2007-965258

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Truncated Hemoglobins in Actinorhizal Nodules of Datisca glomerata

K. Pawlowski¹ ^, ² ^, ⁶ , K. R. Jacobsen³ , N. Alloisio⁴ , R. Ford Denison³ ^, ⁷ , M. Klein³ ^, ⁸ , J. D. Tjepkema⁵ , T. Winzer¹ , A. Sirrenberg¹ ^, ⁹ , C. Guan² ^, ¹⁰ , A. M. Berry³

¹Albrecht von Haller Institute for Plant Sciences, Department of Plant Biochemistry, Göttingen University, 37077 Göttingen, Germany
²Department of Molecular Biology, Wageningen University, 6703 HA Wageningen, The Netherlands
³Department of Plant Sciences, University of California, Davis, CA 95616, USA
⁴Laboratoire d'Ecologie Microbienne, UMR CNRS 5557, Université Claude Bernard Lyon 1, 69622 Villeurbanne Cedex, France
⁵Department of Biological Sciences, University of Maine, Orono, ME 04469-5722, USA
⁶Present address: Department of Botany, Stockholm University, 10691 Stockholm, Sweden
⁷Present address: Ecology, Evolution, and Behavior, University of Minnesota, St. Paul, MN 55108, USA
⁸Present address: Department of Plant Biology, Cornell University, Ithaca, NY 14850, USA
⁹Present address: Molecular Phytopathology, Göttingen University, 37077 Göttingen, Germany
¹⁰Present address: Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI 53706, USA

Abstract

Three types of hemoglobins exist in higher plants, symbiotic, non-symbiotic, and truncated hemoglobins. Symbiotic (class II) hemoglobins play a role in oxygen supply to intracellular nitrogen-fixing symbionts in legume root nodules, and in one case (Parasponia sp.), a non-symbiotic (class I) hemoglobin has been recruited for this function. Here we report the induction of a host gene, dgtrHb1, encoding a truncated hemoglobin in Frankia-induced nodules of the actinorhizal plant Datisca glomerata. Induction takes place specifically in cells infected by the microsymbiont, prior to the onset of bacterial nitrogen fixation. A bacterial gene (Frankia trHbO) encoding a truncated hemoglobin with O₂-binding kinetics suitable for the facilitation of O₂ diffusion ([Tjepkema et al., 2002]) is also expressed in symbiosis. Nodule oximetry confirms the presence of a molecule that binds oxygen reversibly in D. glomerata nodules, but indicates a low overall hemoglobin concentration suggesting a local function. Frankia TrHbO is likely to be responsible for this activity. The function of the D. glomerata truncated hemoglobin is unknown; a possible role in nitric oxide detoxification is suggested.

Key words

Nitrogen-fixing root nodules - leghemoglobin - hemoglobin - nitric oxide - glb3 - trHb.

Full Text

References

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K. Pawlowski

Albrecht von Haller Institute for Plant Sciences
Department of Plant Biochemistry
Göttingen University

37077 Göttingen

Germany

Email: pawlowski@botan.su.se

Editor: T. Bisseling