Planta Medica, Table of Contents Planta Med 2007; 73(6): 578-584DOI: 10.1055/s-2007-967187 Natural Product Chemistry Original Paper © Georg Thieme Verlag KG Stuttgart · New York HPLC-SPE-NMR Characterization of Sesquiterpenes in an Antimycobacterial Fraction from Warburgia salutaris Cailean Clarkson1 , Eliya V. Madikane2 , 3 , Steen Honoré Hansen4 , Peter J. Smith2 , Jerzy W. Jaroszewski1 1Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark 2Department of Medicine, University of Cape Town, Cape Town, South Africa 3Department of Pharmacology, University of Oxford, Oxford, United Kingdom 4Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark Recommend Article Abstract Buy Article Abstract HPLC-SPE-NMR analysis of a chemically complex antimycobacterial fraction of Warburgia salutaris allowed the rapid identification of seven new and four known drimane- and coloratane-type sesquiterpenes. This recently introduced hyphenated technique is therefore a highly valuable tool for elucidation of the chemistry of biologically active fractions of natural origin. Key words Warburgia salutaris - Canellaceae - HPLC-SPE-NMR - sesquiterpenoids - drimanes - coloratanes - mycobacteria Full Text References References 1 Newman D J, Cragg G M, Snader K M. Natural products as sources of new drugs over the period 1981 - 2002. J Nat Prod. 2003; 66 1022-37. 2 Koehn F E, Carter G T. The evolving role of natural products in drug discovery. Nat Rev Drug Discov. 2005; 4 206-20. 3 Butler M S. Natural products to drugs: natural product derived compounds in clinical trials. Nat Prod Rep. 2005; 22 162-95. 4 Rabe T, van Staden J. Isolation of an antibacterial sesquiterpenoid from Warburgia salutaris . J Ethnopharmacol. 2000; 73 171-4. 5 Wube A A, Bucar F, Gibbons S, Asres K. Sesquiterpenes from Warburgia ugandensis and their antimycobacterial acitivity. Phytochemistry. 2005; 66 2309-15. 6 Jansen B JM, de Groot A. Occurrence, biological acitivity and synthesis of drimane sesquiterpenoids. Nat Prod Rep. 2004; 21 449-77. 7 Madikane E V, Bhakta S, Smith P J, Sim E. 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