Suitability of Biomaterials for Cell Delivery in Vitro

Abstract

Re-epithelialization of cutaneous wounds is a coordinated process of proliferation and migration of keratinocytes from the wound. Some attempts have been taken to stimulate wound healing by topical application of various dressings. Cell seeded carriers have been used for accelerating reepithelialization. In vitro we tested a biodegradable D,L-lactide/caprolactone material, Suprathel^®, for cell compatibility. Seeding efficacy (SE) as well as doubling time (DT) of a keratinocyte cell line (HaCaT) and fibroblasts cultured on Suprathel were measured and compared to Biobrane and tissue culture plastic. SE for HSF on Biobrane was 0.20 and on Suprathel 1.23. SE for HaCaT on Biobrane was 0.31 and on Suprathel 1.04 with a SE for tissue culture plastic set as 1. DT between day 2 and 4 for HaCaT on Biobrane was 42.44 h, on Suprathel 40.89 h and on tissue culture plastic 30.57 h. At all time points overall absolute numbers of HaCaT were significantly (p<0.05) higher on Suprathel as compared to Biobrane. DT between day 2 and 4 for HSF on Biobrane was 42.36 h, on Suprathel 40.19 h and on tissue culture plastic 30.91 h. Overall absolute numbers of HaCaT were significantly (p<0.05) higher on Suprathel as compared to Biobrane from day 2 until day 6. Suprathel stimulated cell proliferation similar to Biobrane. In addition the material may be used as a carrier material for cell delivery to wounds.

Keratinocytes - fibroblasts - wound - dressing

References

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