Sage Extract Rich in Phenolic Diterpenes Inhibits Ultraviolet-Induced Erythema in Vivo

Abstract

The leaves of sage (Salvia officinalis L., Lamiaceae) contain high amounts of phenolic diterpenes such as carnosol and carnosic acid. These compounds display antioxidant and anti-inflammatory effects in vitro. Here, we have investigated the anti-inflammatory potency of a sage extract (SE) rich in phenolic diterpenes in vivo using the ultraviolet (UV) erythema test. In a prospective randomised double-blind placebo-controlled study, test areas on the backs of 40 healthy volunteers were irradiated with the 1.5-fold minimal erythema dose. Subsequently, the test areas were treated occlusively with 2 % SE in a hydrophilic ointment, compared to 1 % hydrocortisone and 0.1 % betamethasone as positive controls, and the vehicle alone as placebo. Erythema values were measured photometrically prior to irradiation and after 48 hours. Compared to placebo, SE significantly reduced the ultraviolet-induced erythema, to a similar extent as hydrocortisone. These data suggest that SE might be useful in the topical treatment of inflammatory skin diseases.

References

• 1 Miura K, Kikuzaki H, Nakatani N. Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method.  J Agric Food Chem. 2002;  27 845-51.
• 2 Lo A H, Liang Y C, Lin-Shiau S Y, Ho C T, Lin J K. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-kappaB in mouse macrophages.  Carcinogenesis. 2002;  23 983-91.
• 3 Jocher A, Kessler S, Hornstein S, Schulte-Mönting J, Schempp C M. The UV erythema test as a model to investigate the anti-inflammatory potency of topical preparations - reevaluation and optimization of the method.  Skin Pharmacol Physiol. 2005;  18 234-40.
• 4 Masuda T, Inaba Y, Tomomi M, Takeda Y, Tamura H, Yamaguchi H. Recovery mechanism of the antioxidant activity from carnosic acid quinone, an oxidized sage and rosemary antioxidant.  J Agric Food Chem. 2002;  50 5863-9.
• 5 Huang S C, Ho C T, Lin-Shiau S Y, Lin J K. Carnosol inhibits the invasion of B16/F10 mouse melanoma cells by suppressing metalloproteinase-9 through down-regulating nuclear factor-kappa B and c-Jun.  Biochem Pharmacol. 2005;  69 221-32.
• 6 Rau O, Wurglics M, Paulke A, Zitzkowski J, Meindl N, Bock A. et al . Carnosic acid and carnosol, phenolic diterpene compounds of the labiate herbs rosemary and sage, are activators of the human peroxisome proliferator-activated receptor gamma.  Planta Med. 2006;  72 881-7.
• 7 Kippenberger S, Loitsch S M, Grundmann-Kollmann M, Simon S, Dang T A, Hardt-Weinelt K. et al . Activators of peroxisome proliferator-activated receptors protect human skin from ultraviolet-B-light-induced inflammation.  J Invest Dermatol. 2001;  117 1430-6.