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Planta Med 2007; 73(11): 1169-1175
DOI: 10.1055/s-2007-981586
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Radioprotective Properties of the Phytochemically Characterized Extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on Human Lymphocytes in vitro

Andreja Leskovac1 , Gordana Joksic1 , Teodora Jankovic2 , Katarina Savikin2 , Nebojsa Menkovic2
  • 1Vinca Institute of Nuclear Sciences, Belgrade, Serbia
  • 2Institute for Medicinal Plant Research ”Dr. Josif Pancic”, Belgrade, Serbia
Further Information

Publication History

Received: April 19, 2007 Revised: June 28, 2007

Accepted: July 9, 2007

Publication Date:
31 August 2007 (online)

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Abstract

The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. & Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of 60Co γ-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were γ-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23 % and 13.24 %, respectively), reduced levels of lipid peroxidation products (50.04 % and 13.18 %, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88 % and 35.56 %, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents.

Key words

Crataegus monogyna - Rosaceae - Cornus mas - Cornaceae - Gentianella austriaca - Gentianaceae - polyphenols - radioprotection

References

  • 1 Cos P, Calomme M, Sindambiwe J B, De Bruyne T, Cimanga K, Pieters L. et al . Cytotoxicity and lipid peroxidation-inhibiting activity of flavonoids.  Planta Med. 2001;  67 515-9.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 2 Nijveldt R J, van Nood E, van Hoorn D EC, Boelens P G, van Norren K, van Leeuwen P AM. Flavonoids: a review of probable mechanisms of action and potential applications.  Am J Clin Nutr. 2001;  74 418-25.
    PubMedGoogle Scholar
  • 3 Schuchter L M, Glik J. The current status of WR-2721 (amifostine): a chemotherapy and radiation therapy protector.  Biol Ther Cancer. 1993;  3 1-10.
    PubMedGoogle Scholar
  • 4 Sagar R K, Chawla R, Arora R, Singh S, Krishna B, Sharma R K. et al . Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage.  Planta Med. 2006;  72 114-20.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 Arora R, Gupta D, Chawla R, Sagar R, Sharma A, Kumar R. et al . Radioprotection by plant products: present status and future prospects.  Phytother Res. 2005;  19 1-22.
    CrossrefPubMedGoogle Scholar
  • 6 Nadinic E, Gorzalczany S, Rojo A, van Baren C, Debenedetti S, Acevedo C. Topical anti-inflammatory activity of Gentianella achalensis .  Fitoterapia. 1999;  70 166-71.
    CrossrefPubMedGoogle Scholar
  • 7 Natarajan A T, Boei J J, Darroudi F, Van Diemen P C, Dulout F, Hande M P. et al . Current cytogenetic methods for detecting exposure and effects of mutagens and carcinogens.  Environ Health Perspect. 1996;  104 445-8.
    CrossrefPubMedGoogle Scholar
  • 8 West C M. Invited review: intrinsic radiosensitivity as a predictor of patient response to radiotherapy.  Br J Radiol. 1995;  68 827-37.
    PubMedGoogle Scholar
  • 9 Singleton V L, Rossi J A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents.  Am J Enol Vitic. 1965;  16 144-58.
    PubMedGoogle Scholar
  • 10 European Pharmacopoeia. 5th Edition, Vol. 2 Strasbourg; Council of Europe 2005: 1712-3.
    Google Scholar
  • 11 Jankovic T, Krstic D, Aljancic I, Savikin-Fodulovic K, Menkovic N, Vajs V. et al . Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro.  Biochem Syst Ecol. 2005;  33 729-35.
    PubMedGoogle Scholar
  • 12 Law on health care. Official Gazette of the Republic of Serbia. Vol. 107 Parliament of the Republic of Serbia 2005: 112-61.
    Google Scholar
  • 13 International Atomic Energy Agency: Cytogenetic analysis for Radiation Dose Assessment. Technical Series No. 405. Vienna; IAEA 2001.
    Google Scholar
  • 14 Fenech M. The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations.  Mutat Res. 1993;  285 35-44.
    CrossrefPubMedGoogle Scholar
  • 15 Surralles J, Xamena N, Creus A, Marcos R. The suitability of the micronucleus assay in human lymphocytes as a new biomarker of excision repair.  Mutat Res. 1995;  342 43-59.
    PubMedGoogle Scholar
  • 16 Aruoma O I, Halliwell B, Laughton M J, Quinlan G J, Gutteridge J M. The mechanism of initiation of lipid peroxidation. Evidence against a requirement for an iron(II)-iron(III) complex.  Biochem J. 1989;  258 617-20.
    PubMedGoogle Scholar
  • 17 Lowry O H, Rosebrough N J, Farr A L, Randall R J. Protein measurement with the Folin phenol reagent.  J Biol Chem. 1951;  193 265-75.
    PubMedGoogle Scholar
  • 18 Crompton N E, Ozsahin M. A versatile and rapid radiosensitivity assay of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity.  Radiat Res. 1997;  147 55-60.
    CrossrefPubMedGoogle Scholar
  • 19 Thresiamma K C, George J, Kuttan R. Protective effect of curcumin, ellagic acid and bixin on radiation induced genotoxicity.  J Exp Clin Cancer Res. 1998;  17 431-4.
    PubMedGoogle Scholar
  • 20 Gebhardt R. Oxidative stress, plant-derived antioxidants and liver fibrosis.  Planta Med. 2002;  68 289-96.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 21 Laupattarakasem P, Houghton P J, Hoult J RS. Anti-inflammatory isoflavonoids from the stems of Derris scandens .  Planta Med. 2004;  70 496-501.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Seghrouchni I, Drai J, Bannier E, Riviere J, Calmard P, Garcia I. et al . Oxidative stress parameters in type I, type II and insulin-treated type 2 diabetes mellitus; insulin treatment efficiency.  Clin Chim Acta. 2002;  321 89-96.
    CrossrefPubMedGoogle Scholar
  • 23 Das U. A radical approach to cancer.  Med Sci Monit. 2002;  8 79-92.
    PubMedGoogle Scholar
  • 24 Umegaki K, Ikegami S, Inoue K, Ichikawa T, Kobayashi G, Soeno N. et al . Beta carotene prevents X-ray induction of micronuclei in human lymphocytes.  Am J Clin Nutr. 1994;  59 409-12.
    PubMedGoogle Scholar
  • 25 Vrinda B, Uma Devi P. Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin.  Mutat Res. 2001;  498 39-46.
    PubMedGoogle Scholar
  • 26 Jagetia G C, Venkatesh P, Baliga M S. Evaluation of the radioprotective effect of Aegle marmelos (L.) Correa in cultured human peripheral blood lymphocytes exposed to different doses of gamma-radiation: a micronucleus study.  Mutagenesis. 2003;  18 387-93.
    CrossrefPubMedGoogle Scholar
  • 27 Wen X, Walle T. Methylated flavonoids have greatly improved intestinal absorption and metabolic stability.  Drug Metab Dispos. 2006;  34 786-92.
    CrossrefPubMedGoogle Scholar

Andreja Leskovac

Vinca Institute of Nuclear Sciences

POB 522

11001 Belgrade

Serbia

Phone: +38-1-11-245-3967

Fax: +38-1-11-244-7207

Email: andreja@vin.bg.ac.yu