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Planta Med 2007; 73(11): 1208-1213
DOI: 10.1055/s-2007-981591
Natural Product Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Anti-Inflammatory Ergostanes from the Basidiomata of Antrodia salmonea

Chien-Chang Shen1 , Yea-Hwey Wang2 [*] , Tun-Tschu Chang3 [*] , Lie-Chwen Lin1 , Ming-Jaw Don1 , Yu-Chang Hou4 , Kuo-Tong Liou5 , Shiou Chang4 , Wen-Yen Wang4 , Han-Chieh Ko1 , Yuh-Chiang Shen1
  • 1National Research Institute of Chinese Medicine, Taipei, Taiwan
  • 2Department of Pharmacy, Taipei Veterans General Hospital, Taipei, Taiwan
  • 3Division of Forest Protection, Taiwan Forestry Research Institute, Taipei, Taiwan
  • 4Departments of Surgery and Chinese Medicine, Hsinchu and Taoyuan General Hospital, Department of Health, Taiwan
  • 5Graduate Institute of Sport Coaching Science, Chinese Culture University, Taipei, Taiwan
Further Information

Publication History

Received: October 27, 2006 Revised: July 11, 2007

Accepted: July 16, 2007

Publication Date:
31 August 2007 (online)

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Abstract

Three new anti-oxidative ergostanes, methyl antcinate L (1), antcin M (2), and methyl antcinate K (3), together with nine additional known compounds, 3-ketodehydrosulphurenic acid, sulphurenic acid, dehydrosulphurenic acid, 3β,15α-dihydroxylanosta-7,9(11),24-trien-21-oic acid, zhankuic acid A, zhankuic acid B, zhankuic acid C, antcin C, and antcin K were isolated from the basidiomata of Antrodia salmonea, a newly identified species of Antrodia (Polyporaceae) in Taiwan. These three new compounds were identified as methyl 3α,7α,12α-trihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oate (1), 3α,12α-dihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oic acid (2), and methyl 3α,4β,7β-trihydroxy-4α-methylergosta-8,24(29)-dien-11-on-26-oate (3) by spectroscopic analysis. We studied their antioxidative potential on the production of reactive oxygen species and nitric oxide (NO) in neutrophils and microglial cells, respectively. Compounds 1 - 3 displayed potent antioxidative activity with IC50 values of around 2.0 - 8.8 μM that was partially due to inhibition (6 - 67 %) of NADPH oxidase activity but not through direct radical-scavenging properties. Compounds 1 - 3 also inhibited NO production with IC50 values of around 1.7 - 16.5 μM and were more potent than a non-specific NOS inhibitor. We conclude that these three new compounds 1, 2, and 3 exhibit anti-inflammatory activities in activated inflammatory cells.

Key words

Polyporaceae - Antrodia salmonea - ergostane - lanostane - microglial cell - neutrophils - reactive oxygen species - nitric oxide (NO)

References

1 Y. H. Wang and T. T. Chang contributed to this work equally

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Yuh-Chiang Shen, PhD

National Research Institute of Chinese Medicine

No. 155-1, Sec. 2, Li-Nong Street

Taipei 112

Taiwan

R.O.C.

Phone: +886-2-2820-1999 ext 9101

Fax: +886-2-2826-4266

Email: yuhcs@nricm.edu.tw