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Thorac Cardiovasc Surg 2008; 56(7): 406-411
DOI: 10.1055/s-2008-1038731
Original Cardiovascular

© Georg Thieme Verlag KG Stuttgart · New York

Can Dantrolene Protect Spinal Cord against Ischemia/Reperfusion Injury? An Experimental Study

C. U. Kocogullari1 , M. Emmiler1 , M. Cemek2 , O. Sahin3 , A. Aslan4 , E. Ayva1 , L. Tur2 , M. E. Buyukokuroglu5 , I. Demirkan6 , A. Cekirdekci1
  • 1Department of Cardiovascular Surgery, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
  • 2Department of Chemistry, Afyon Kocatepe University Arts and Science Faculty, Afyon, Turkey
  • 3Department of Pathology, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
  • 4Department of Neurosurgery, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
  • 5Department of Pharmacology, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
  • 6Department of Surgery, Afyon Kocatepe University Veterinary Faculty, Afyon, Turkey
Further Information

Publication History

received March 10, 2008

Publication Date:
22 September 2008 (online)

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Abstract

Background: The aim of this experimental study was to investigate the possible protective effect of dantrolene on neuronal injury induced by aortic ischemia/reperfusion (I/R). Methods: Nineteen rabbits were divided into three groups: sham (group 1, n = 5, no I/R), control (group 2, n = 7, only I/R) and dantrolene (group 3, n = 7, dantrolene + I/R). Abdominal aortic occlusion between the renal arteries and iliac bifurcations was carried out for 30 min. The spinal cord functions of the subjects were assessed using the Tarlov Scale. Blood and cord tissue samples were taken for biochemical and histopathological evaluation. Results: Tarlov scores in group 3 were significantly higher than in group 2 (p < 0.05). In group 3, the MDA levels of the spinal cord decreased significantly compared to those of group 2 (p < 0.05). In rabbits with I/R (group 2), the GSH levels of the spinal cord decreased significantly compared to those of group 1 (p < 0.01), but dantrolene pretreatment significantly prevented a decrease in GSH levels. Histopathological examination showed that group 3 had less vascular proliferation, hemorrhage, edema and neuron loss than group 2. Conclusions: It was concluded that dantrolene applied after ischemia might help protect the spinal cord against ischemia/reperfusion injury.

Key words

aortic disease - cardiovascular surgery - aortic surgery

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Ass. Prof. Cevdet Ugur Kocogullari

Department of Cardiovascular Surgery
Afyon Kocatepe University Medical Faculty

Dervispasa Mah. Dr. Mahmut Hazar Caddesi Buse Apt. No: 43A Blok B B Giris D:4

02300 Afyon

Turkey

Phone: + 90 27 22 14 76 14

Fax: + 90 27 22 46 33 22

Email: cevdetkoc@hotmail.com