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Thorac Cardiovasc Surg 2008; 56(8): 441-448
DOI: 10.1055/s-2008-1038879
Basic Science

© Georg Thieme Verlag KG Stuttgart · New York

Different Biological Properties of Circulating and Bone Marrow Endothelial Progenitor Cells in Acute Myocardial Infarction Rats

Z. Xin1 , W. Meng1 , H. Ya-ping1 , Z. Wei1
  • 1The Division of Cardiology, Shanghai Sixth Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China
Further Information

Publication History

received March 9, 2008

Publication Date:
14 November 2008 (online)

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Abstract

Background: Many studies have shown that endothelial progenitor cell (EPC) can enhance the neovascularization of the ischemic myocardium. Peripheral blood and bone marrow are the most convenient resources for EPC.

Objective: The aim of the study was to investigate, in vitro and in vivo, the different biological properties between circulating EPC (CEPC) and bone marrow EPC (BM‐EPC) of AMI rats.

Methods: The proliferative, migrative, adherent and angiogenic properties were investigated in vitro. 1 × 106 CEPCs, 1 × 106 BM-EPCs and medium (EBM-2) were injected in the myocardium of AMI rats. Echocardiography, regional myocardial blood flow (RMBF), capillary density and Y chromosome fluorescence in situ hybridization (FISH) were performed at 4 weeks after transplantation.

Results: The CEPCs had higher proliferative, migrative, adherent capabilities and lower senescent ratio, could adhere more quickly to fibronectin than BM-EPCs. CEPCs could form capillary-like structures whereas BM-EPCs did not show similar structures on Matrigel. In vivo, transplanted CEPCs and BM-EPCs were found in cardiac tissue by FISH. CEPCs treatment led to a better cardiac function, RMBF and capillary density than BM-EPCs.

Conclusion: Different biological properties were observed between CEPCs and BM-EPCs. Autologous CEPCs are more suitable for the AMI rat.

Key words

endothelial progenitor cells - bone marrow - circulating - acute myocardial infarction

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Prof. Wei Meng

Shanghai Sixth Hospital, Shanghai JiaoTong University School of Medicine
The Division of Cardiology

No. 600, Yishan Road

200233 Shanghai

China

Fax: + 86 21 64 70 19 32

Email: drweimeng@hotmail.com