Increased platelet activation in young Zucker rats with impaired glucose tolerance is improved by acarbose

Andreas Schäfer; Julian Widder; Martin Eigenthaler; Hilmar Bischoff; Georg Ertl; Johann Bauersachs

doi:10.1160/TH04-02-0118

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2004; 92(01): 97-103
DOI: 10.1160/TH04-02-0118

Platelets and Blood Cells

Schattauer GmbH

Increased platelet activation in young Zucker rats with impaired glucose tolerance is improved by acarbose

Authors

Andreas Schäfer^*

¹Department of Internal Medicine, Cardiology, University of Würzburg,Würzburg, Germany
Julian Widder^*

¹Department of Internal Medicine, Cardiology, University of Würzburg,Würzburg, Germany
Martin Eigenthaler

²Institute for Clinical Biochemistry and Pathobiochemistry, University of Würzburg,Würzburg, Germany
Hilmar Bischoff

³Bayer AG,Wuppertal, Germany
Georg Ertl

¹Department of Internal Medicine, Cardiology, University of Würzburg,Würzburg, Germany
Johann Bauersachs

¹Department of Internal Medicine, Cardiology, University of Würzburg,Würzburg, Germany

Abstract

Summary

Patients with diabetes display increased platelet activation. Recent data show a markedly increased risk for cardiovascular events already in pre-diabetic individuals with impaired glucose tolerance (IGT). We investigated whether IGT is associated with platelet activation. Blood samples were collected from young lean (control) and obese Zucker rats, an established model of IGT, after single oral application of sucrose (4g·kg-1). Platelet-bound fibrinogen and platelet surface-expression of P-selectin were assessed as indices of platelet activation using flow cytometry. In lean Zucker rats, acute sucrose application induced fibrinogen-binding and P-selectin surface-expression, which was prevented by co-administration of acarbose (10mg·kg-1). In obese Zucker rats, platelet activation was already maximally increased under baseline conditions with no significant increase after sucrose application. Chronic treatment with acarbose (15mg·kg-1·day-1) significantly reduced platelet activation in these animals. Acute ingestion of sucrose induces platelet activation which is prevented by acarbose. IGT is associated with marked platelet activation that can be reduced by chronic administration of acarbose.The positive modulation of platelet activation by acarbose may contribute to the reduction of cardiovascular events in patients with IGT.

Keywords

Platelet activation markers - diabetes / metabolic disorders - animal models

Full Text

References

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