Differential expression of tissue factor mRNA and protein expression in murine sepsis

Vivian de Waard; Hjalmar R. Hansen; Henri H. M. Spronk; Janneke J. Timmerman; Hans Pannekoek; Sandrine Florquin; Pieter H. Reitsma; Hugo ten Cate

doi:10.1160/TH05-07-0512

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2006; 95(02): 348-353
DOI: 10.1160/TH05-07-0512

Animal Models

Schattauer GmbH

Differential expression of tissue factor mRNA and protein expression in murine sepsis

The role of the granulocyte revisited

Vivian de Waard

¹Department of Biochemistry, Academic Medical Center, Amsterdam

,

Hjalmar R. Hansen

²Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam

,

Henri H. M. Spronk

⁴Laboratory for Clinical Thrombosis and Haemostasis, Department of Internal Medicine and Cardiovascular Research Institute, University of Maastricht, The Netherlands

,

Janneke J. Timmerman

²Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam

,

Hans Pannekoek

¹Department of Biochemistry, Academic Medical Center, Amsterdam

,

Sandrine Florquin

³Department of Pathology, Academic Medical Center, Amsterdam

,

Pieter H. Reitsma

²Laboratory for Experimental Internal Medicine, Academic Medical Center, Amsterdam

,

Hugo ten Cate

⁴Laboratory for Clinical Thrombosis and Haemostasis, Department of Internal Medicine and Cardiovascular Research Institute, University of Maastricht, The Netherlands

› Author Affiliations

Abstract

Summary

Tissue factor (TF) is a transmembrane protein, which is essential for initiation of the coagulation cascade. TF has been reported to play an important role in the progression of endotoxin (lipopolysaccharide, LPS)-mediated endotoxemia, being induced in numerous tissues, such as kidney, spleen and lung. We developed and validateda rabbit anti-murine TF peptide antiserum to localize TF protein in a murine sepsis model. TF protein distribution was compared to localization of TF mRNA and fibrin deposits, the ultimate resultant of procoagulant TF activity. Evident LPSmediated TF mRNA induction was observed in the tubular area at the cortico-medullar junction in the kidney, and TF activity was increased after 6 hours of endotoxemia. In the spleen, however, TF mRNA was induced in the interfollicular region upon LPS injection, corresponding to increased TF protein in the same area. The clusters of TF-protein positive cells in the spleen are predominantly granulocytes, but no TF mRNA expression was observed within these cells. Based on these observations and the presence of TF-protein positive granulocytes after splenectomy, we hypothesize that granulocytes take-up TF for transport to other locations in order to initiate fibrin formation or to induce pronflammatory gene expression upon interaction with factor VIIa.

Keywords

Tissue factor - coagulation - sepsis - inflammation - granulocytes

Full Text

References

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