Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

Petra Censarek; Anett Bobbe; Maria Grandoch; Karsten Schrör; Artur-Aron Weber

doi:10.1160/TH06-09-0524

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2007; 97(01): 11-14
DOI: 10.1160/TH06-09-0524

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Alternatively spliced human tissue factor (asHTF) is not pro-coagulant

Petra Censarek^*

¹Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany

,

Anett Bobbe^*

¹Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany

,

Maria Grandoch

²Institut für Pharmakologie, Universität Duisburg-Essen, Universitätsklinikum, Germany

,

Karsten Schrör

¹Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany

,

Artur-Aron Weber

¹Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany

› Author Affiliations

Abstract

Summary

It has been proposed that alternatively-spliced human tissue factor (asHTF) is pro-coagulant. We have evaluated the function of asHTF in a mammalian expression system. Full-length human tissue factor (HTF) and asHTF were cloned from smooth muscle cells and over-expressed in HEK293 cells. As expected, a marked pro-coagulant activity (FX activation, thrombin generation) was observed on the surface, in lysates, and on microparticles from HTF transfected cells. In contrast, no pro-coagulant activity of as HTF was observed.

Keywords

Coagulation factors - tissue factor / factor VII - thrombosis

Full Text

References

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