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Thromb Haemost 2011; 105(05): 776-782
DOI: 10.1160/TH10-12-0771
Theme Issue Article
Schattauer GmbH

Imaging fibrin formation and platelet and endothelial cell activation in vivo

Lola Bellido-Martin   *
1   Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachussetts, USA
,
Vivien Chen   *
1   Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachussetts, USA
,
Reema Jasuja   *
1   Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachussetts, USA
,
Bruce Furie
2   Harvard Medical School, Boston, Massachussetts, USA
,
Barbara C. Furie
2   Harvard Medical School, Boston, Massachussetts, USA
› Author Affiliations Financial support:This work was supported by grants from the National Institutes of Health to B.F. and B.C.F. R.J. is a recipient of a fellowship from the American Heart Association and V.M.C. is a recipient of a grant from the National Health and Medical Research Council of Australia.
Further Information

Publication History

Received: 04 December 2010

Accepted after minor revision: 04 February 2011

Publication Date:
28 November 2017 (online)

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Summary

Over the past six decades research employing in vitro assays has identified enzymes, cofactors, cell receptors and associated ligands important to the haemostatic process and its regulation. These studies have greatly advanced our understanding of the molecular and cellular bases of haemostasis and thrombosis. However, in vitro assays cannot simultaneously reproduce the interactions of all of the components of the haemostatic process that occur in vivo nor do they reflect the importance of haemodynamic factors resulting from blood flow. To overcome these limitations investigators have increasingly turned to animal models of haemostasis and thrombosis. In this article we describe some advances in the visualisation of platelet and endothelial cell activation and blood coagulation in vivo and review what we have learned from our intravital microscopy experiments using primarily the laser-induced injury model for thrombosis.

Keywords

Animal models - thrombosis - intravital imaging

* These authors contributed equally to the preparation of this review.


 

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