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Thromb Haemost 2012; 108(04): 592-598
DOI: 10.1160/TH12-02-0097
Theme Issue Article
Schattauer GmbH

Analytical challenges and technical limitations in assessing circulating MiRNAs

Anna Zampetaki
1   King’s British Heart Foundation Centre, King’s College London, London, UK
,
Manuel Mayr
1   King’s British Heart Foundation Centre, King’s College London, London, UK
› Author Affiliations
Further Information

Publication History

Received: 17 February 2012

Accepted after minor revision: 17 April 2012

Publication Date:
29 November 2017 (online)

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Summary

MiRNAs are emerging as promising biomarkers in cardiovascular diseases and may constitute a novel mechanism of intercellular communication. Accurate quantification of circulating miRNAs is essential. A variety of technological approaches and platforms have been developed with increased sensitivity and specificity for the detection and quantification of circulating miRNAs. In this review, we focus on the technical aspects and discuss the analytical challenges in profiling circulating miRNAs.

Keywords

microRNA - biomarker - cardiovascular - diabetes
 

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