Analytical challenges and technical limitations in assessing circulating MiRNAs

Anna Zampetaki; Manuel Mayr

doi:10.1160/TH12-02-0097

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2012; 108(04): 592-598
DOI: 10.1160/TH12-02-0097

Theme Issue Article

Schattauer GmbH

Analytical challenges and technical limitations in assessing circulating MiRNAs

Anna Zampetaki

¹King’s British Heart Foundation Centre, King’s College London, London, UK

,

Manuel Mayr

¹King’s British Heart Foundation Centre, King’s College London, London, UK

› Author Affiliations

Abstract

Summary

MiRNAs are emerging as promising biomarkers in cardiovascular diseases and may constitute a novel mechanism of intercellular communication. Accurate quantification of circulating miRNAs is essential. A variety of technological approaches and platforms have been developed with increased sensitivity and specificity for the detection and quantification of circulating miRNAs. In this review, we focus on the technical aspects and discuss the analytical challenges in profiling circulating miRNAs.

Keywords

microRNA - biomarker - cardiovascular - diabetes

Full Text

References

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