Dietary omega-3 alpha-linolenic acid does not prevent venous thrombosis in mice

Martin F. Reiner; Kimberly Martinod; Simona Stivala; Gianluigi Savarese; Giovanni G. Camici; Thomas F. Lüscher; Denisa D. Wagner; Jürg H. Beer

doi:10.1160/TH14-03-0200

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2015; 113(01): 177-184
DOI: 10.1160/TH14-03-0200

Stroke, Systemic or Venous Thromboembolism

Schattauer GmbH

Dietary omega-3 alpha-linolenic acid does not prevent venous thrombosis in mice

Authors

Martin F. Reiner^*

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland

²Department of Internal Medicine, Cantonal Hospital of Baden, Switzerland
Kimberly Martinod^*

³Program in Cellular and Molecular Medicine, Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Simona Stivala

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland

²Department of Internal Medicine, Cantonal Hospital of Baden, Switzerland
Gianluigi Savarese

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland
Giovanni G. Camici

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland
Thomas F. Lüscher

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland

⁵Department of Cardiology, University Heart Center, University Hospital Zurich, Switzerland
Denisa D. Wagner

³Program in Cellular and Molecular Medicine, Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA

⁴Division of Hematology/Oncology, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
Jürg H. Beer

¹Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, Schlieren, Switzerland

²Department of Internal Medicine, Cantonal Hospital of Baden, Switzerland

Abstract

Summary

Venous thromboembolism (VTE) is a leading cause of cardiovascular death. Omega-3 fatty acids (n-3 FA) exhibit protective effects against cardiovascular disease. Others and our group have reported that the plant-derived n-3 FA alpha-linolenic acid (ALA) displays antiinflammatory, anticoagulant and antiplatelet effects, thereby reducing atherosclerosis and arterial thrombosis in mice fed a high ALA diet. Since procoagulant factors such as tissue factor and fibrin as well as platelets and leukocytes are crucially involved in the development of VTE, we investigated possible protective effects of dietary ALA on venous thrombus formation in a mouse model of stenosis- and furthermore, in a mouse model of endothelial injury-induced venous thrombosis. Four week old C57BL/6 mice underwent four weeks of high (7.3g%) or low ALA (0.03g%) treatment before being exposed to inferior vena cava (IVC) stenosis for 48 hours or laser injury of the endothelium of the internal jugular vein (IJV). Thrombus generation frequency, thrombus size and composition (IVC stenosis group) and time to thrombus formation (endothelial injury group) were assessed. In addition, plasma glycocalicin, a marker of platelet activation, platelet P-selectin and activated integrin expression as well as plasma thrombin generation was determined, but did not reveal any significant differences between he groups. Despite its protective properties against arterial thrombus formation, dietary ALA did not protect against venous thrombosis neither in the IVC stenosis nor the endothelial injury model, further indicating that the biological processes involved in arterial and venous thrombosis are different.

Keywords

Alpha-linolenic acid - cardiovascular disease - eep vein thrombosis - omega-3 fatty acids - venous thromboembolism

Full Text

References

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