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Tierarztl Prax Ausg K Kleintiere Heimtiere 2017; 45(04): 246-252
DOI: 10.15654/TPK-160059
Original Article
Schattauer GmbH

Comparative aspects of blood coagulation measurements in various wild and captive bird species

Vergleichende Bestimmung von Gerinnungsparametern in Blutproben verschiedener Wild- und Ziervogelspezies
Vanessa Guddorf
1   Clinic for Small Mammals, Reptiles and Birds, University of Veterinary Medicine Hannover, Hannover, Germany
,
Karl Rohn
2   Institute for Biometry, Epidemiology and Information Processing (IBEI), University of Veterinary Medicine Hannover, Hannover, Germany
,
Norbert Kummerfeld
1   Clinic for Small Mammals, Reptiles and Birds, University of Veterinary Medicine Hannover, Hannover, Germany
,
Reinhard Mischke
3   Clinic for Small Animals, University of Veterinary Medicine Hannover, Hannover, Germany
› Author Affiliations
Further Information

Publication History

Received: 12 January 2016

Accepted after revision: 24 January 2017

Publication Date:
10 January 2018 (online)

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Summary

Objective: Information about the influence of species variety or diseases on coagulation values in avian blood is rare. The aim of this study was to assess the influence of species on measurements of coagulation parameters in avian plasma samples using commercially available reagents and to investigate potential influences of selected diseases on clotting times. Material and methods: Prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT) were measured in citrated plasma of healthy individuals collected from eight different wild and captive avian species applying currently commercially available reagents prepared for use in humans, which were preselected in preliminary studies. The same parameters were tested in plasma samples from birds affected by aspergillosis, atherosclerosis, neoplasia and traumata. Results: PT and aPTT showed a high interspecies variety. Irrespective of species, aPTTs were extremely long and partially exceeded the measurement range limit. Minor variations between species were seen in TT measurements. Clotting times obtained from birds affected by aspergillosis, atherosclerosis and neoplasia were not significantly different when compared to healthy birds. Plasma obtained from traumatised individuals showed significantly shorter PT and aPTT than that in healthy birds. Conclusion and clinical relevance: Differences between species must be considered in diagnostic coagulation measurements in avian blood. Regardless of the avian species, aPTT measurements on avian samples appear to be of limited value. Lower PT and aPTT values reflect coagulation activation in traumatised birds.

Zusammenfassung

Gegenstand und Ziel: Es gibt nur wenige Informationen über den Arten einfluss sowie den Einfluss von Krankheiten auf die Gerinnung von Vogelblut. Ziel dieser Studie war, Gerinnungsparameter in Blutproben verschiedener Vogelarten unter Verwendung von kommerziell erhält lichen Reagenzien zu bestimmen, um die Speziesvarietät zu erfassen und einen möglichen Einfluss selektierter Erkrankungen auf die Gerinnungszeiten zu untersuchen. Material und Methoden: Die Prothrombinzeit (PT), die aktivierte partielle Thromboplastinzeit (aPTT) sowie die Thrombinzeit (TT) wurden in Zitratplasma gesunder Individuen von acht verschiedenen Wild- und Ziervogelspezies gemessen. Hierzu dienten aktuell kommerziell verfügbare, in der Humanmedizin eingesetzte Reagenzien, die in einer vorhergehenden Studie vorselektiert wurden. Ferner erfolgte eine Messung dieser Gerinnungsparameter in Plasmaproben von Vögeln, die an Aspergillose, Atherosklerose oder einer Neoplasie erkrankt waren oder unter einem akuten Trauma litten. Ergebnisse: Bei der PT und aPTT zeigte sich eine hohe Speziesdiversität. Unabhängig von der Spezies waren die aPTTs extrem lang und überschritten teilweise die Messgrenze. Hinsichtlich der Thrombinzeit ergaben sich geringere Unterschiede zwischen den Spezies. Zwischen den Gerinnungszeiten der an Aspergillose, Atherosklerose oder Neoplasie erkrankten Vögel und den gesunden Tieren konnten keine signifikanten Unterschiede fest gestellt werden. Die Plasmaproben traumatisierter Vögel wiesen signifikant kürzere PTs und aPTTs auf als die Proben der gesunden Individuen. Schlussfolgerung und klinische Relevanz: Die Unterschiede zwischen den verschiedenen Spezies müssen bei der Gerinnungsdia gnostik berücksichtigt werden. Die limitierte Verwendbarkeit der aPTT in Vogelproben scheint speziesunabhängig vorzuliegen. Die verkürzten PTs und aPTTs der traumatisierten Vögel reflektieren die Aktivierung der Gerinnung.

Keywords

Haemostasis - birds - prothrombin time - activated partial thromboplastin time - thrombin time - species variety

Schlüsselwörter

Hämostase - Vögel - Prothrombinzeit - aktivierte partielle Thromboplastinzeit - Thrombinzeit - Speziesunterschiede
 

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