Experimental neurocysticercosis: absence of IL-4 induces lower encephalitis

ABSTRACT

Neurocysticercosis (NCC) is the most severe clinical manifestation of cysticercosis. One of the factors responsible for its symptomatology is the host inflammatory response. Therefore the influence of interleukin 4 (IL-4) on the induction of encephalitis in experimental NCC was evaluated.

Methods

BALB/c (WT) and BALB/c (IL-4-KO) mice were inoculated intracranially with Taenia crassiceps cysticerci and euthanized at 7, 30, 60 and 90 days later, the encephala removed and histopathologically analyzed.

Results

The absence of IL-4 induced greater parasitism. In the initial phase of the infection, IL-4-KO showed a lower intensity in the inflammatory infiltration of polimorphonuclear cells in the host-parasite interface and intra-parenquimatous edema. The IL-4-KO animals, in the late phase of the infection, showed lower intensity of ventriculomegaly, encephalitis, and meningitis, and greater survival of the parasites in comparison with the WT animals.

Conclusion

The absence of IL-4 induced lower inflammatory infiltration, ventriculomegaly and perivasculitis in experimental NCC.

RESUMO

A Neurocisticercose (NCC) é a manifestação clínica mais severa da cisticercose, e um dos fatores responsáveis pela sintomatologia é a resposta inflamatória do hospedeiro. Desta forma avaliou-se a influência da interleucina 4 (IL-4) na indução de encefalite na NCC experimental.

Métodos

Camundongos das linhagens BALB/c (WT) e BALB/c (IL-4-KO) foram inoculados intracranialmente com cisticercos de Taenia crassiceps e eutanasiados aos 7, 30, 60 e 90 dias após a infecção, os encéfalos foram removidos e analisados histopatologicamente.

Resultados

A ausência da IL-4 induziu um maior parasitismo nos animais. Na fase inicial da infecção os animais IL-4-KO apresentaram menor intensidade tanto de infiltrado inflamatório de polimorfonucleares na interface parasito-hospedeiro quanto de edema intraparenquimatoso. Os animais IL-4-KO, na fase tardia, apresentaram menor intensidade de ventriculomegalia, encefalite, meningite e maior sobrevivência dos cisticercos em relação aos animais WT.

Conclusão

A ausência da IL-4 induz menos infiltrado inflamatório, ventriculomegalia e perivasculite na NCC experimental.

neurocysticercosis - Taenia crassiceps - encephalitis - interleukin-4

neurocisticercose - Taenia crassiceps - encefalite - interleukin-4

Neurocysticercosis (NCC) is the most severe clinical manifestation of cysticercosis and is responsible for up to 29% of the epilepsy cases in Latin America, India and Africa[1],[2]. The NCC symptoms are caused by the host inflammatory response, immune response modulation, location of the parasite, evolutionary stage of the parasite, parasitic burden, as well as the mass effect induced by its growth, which results in a wide clinical and laboratory polymorphism challenging the diagnosis of this disease[3],[4].

Experimental models have been used as important tools in the study and comprehension of the host-parasite relationship in human cysticercosis in several locations including the neurological one[5],[6],[7]. The most-used parasite in the experimental models is Taenia crassiceps cysticercus due to its rapid development cycle and its antigenic similarity with T. solium [7],[8]. The intraperitoneal experimental model is the most common and is very useful in the evaluation of the genetic factors involved in the host’s susceptibility and resistance, with emphasis on the immunological mechanisms[9]. The T. crassiceps intraperitoneal infection induces an initial Th1 response accompanied by large amounts of interferon-gamma, classically activated macrophages (pro-inflammatory or M1), IgG2a antibodies and interleukin-12 (IL-12) leading to a low parasitic burden. Soon afterwards there is a strong Th2 polarization due to the immunoregulatory capacity of the parasite increasing serum levels of IgG and IgE as well as IL-4, IL-13 and IL-5, which inhibits the initial Th1 response and favors the growth of parasitic burden[7],[8],[10],[11].

The experimental models exploring the neurological location of cysticerci are described using Mesocestoides corti [5],[12],[13] and T. crassiceps [6]. The model carried out with M. corti was used to describe the role of defense cells and cytokines in the host-parasite relationship[13], the expression of Toll-like receptors[5], the migration of leucocytes to the central nervous system, the junction complex proteins and the immune system evasion strategies[14]. According to these studies, the immune response in the murine experimental model of NCC is associated with a predominant Th1 immune response directed by gamma-delta T lymphocytes, which arrive at the infection site at the beginning of the infection and remain in it during the whole course of the infection. These cells induce an increase in the pro-inflammatory cytokine production and a greater local cellular response[7], which does not eliminate the parasite, possibly due to its evasion strategies[14]. Therefore there is an increase in the parasitic burden and in the inflammatory cells within extraparenchymatous areas of the encephala, such as ventricles, meninges and subarachnoid space[13]. It is interesting to highlight that a low parasitic burden is able to promote an initial Th1 response that is capable of eliminating the parasite[5]. As the infection progresses, parasites and immune cells reach the brain parenchyma, which increases the inflammation intensity and results in more severe clinical symptoms[13].

Taenia crassiceps is also used as an experimental model for NCC studies due to is antigenic similarities to T. solium cysticerci[8],[15]. It has also already been described that T. crassiceps cysticerci are able to induce NCC in immunocompromised[16] and immunocompetent individuals[17]. In this model, the infected animals presented with tissue alterations and lesions such as encephalitis, perivasculitis, ventriculomegaly, ependymitis, meningitis, microgliosis accompanied by the parasite’s death, and extraparenchymal and parenchymal inflammatory infiltration[6],[18].

Other experimental models have been proposed but instead of using T. crassiceps cysticerci they performed an intracranial inoculation of T. solium oncospheres in rats[19] and in swine[20]. The rat NCC model reported that the infected animals presented with epilepsy, inflammatory infiltration, perivascular infiltrate, angiogeneses, spongy changes and mass effect in the brain tissue[19]. In the swine NCC model, after four months of infection, it was possible to observe calcified cysticerci surrounded by an exacerbated inflammatory response with lymphocyte infiltration and increased inflammatory markers[20].

The BALB/c mice are genetically susceptible to intraperitoneal infection with T. crassiceps cysticerci because they present a Th2 immune profile[7],[9]. However the immunological mechanisms associated with susceptibility or resistance of this mice lineage to T. crassiceps cysticerci are not completely understood.

The aim of this study was to describe and compare the influence of IL-4 in the encephalic inflammatory response to the experimental T. crassiceps NCC infection in two lineages of BALB/c mice - wild type (WT) and IL-4 knockout (IL-4-KO).

METHODS

Maintenance of T. crassiceps

The T. crassiceps cysticerci (ORF strain) have been maintained through intraperitoneal passages in female BALB/c mice[8] in the animal facility of the Tropical Pathology and Public Health Institute (IPTSP) of the Federal University of Goias (UFG) since 2002.

Animals

Two lineages of female BALB/c mice were used: conventional BALB/c also known as WT, and IL-4-KO BALB/c mice that have no gene for IL-4 production. The animals provided by the IPTSP/UFG animal facilities were eight to 12 weeks old and weighed 20 to 30 grams .

In this study the animals were divided into four groups with five mice each as follows: group 1 - WT BALB/c mice inoculated with T. crassiceps cysticerci; group 2 - WT BALB/c mice inoculated with sterile saline solution; groups 3 - IL-4-KO BALB/c mice inoculated with T. crassiceps cysticerci; and group 4 - IL-4-KO BALB/c mice inoculated with a sterile saline solution. This study was approved by the ethics committee for animal use (CEUA/UFG) protocol number 034/09.

Experimental infection

The intracranial infection of the animals was performed as described by Matos-Silva et al[6]. Briefly, the animals were weighed and anesthetized with 0.01ml/g of ketamine 10% and xilazine 2% intraperitoneal injection. After trichotomy of the superior portion of the head and antisepsis with topical iodine, a longitudinal and median incision was made on the skin of the skull with a scalpel. The trepanation orifice was performed with a drill (44.5mm x 2mm) moved by a micromotor (LB100-Beltec) in the topography of the right parietal bone at 3mm from the median line (sagittal suture) and at 3mm posterior to the coronal suture and with 4mm of depth. The infected animals were intracranially inoculated with three to five cysticerci, following which the trepanation orifice was closed with sterile dental alginate and the incision sutured.

Removal of the encephala

At 7, 30, 60 and 90 days post inoculation (DPI) the animals were euthanized with a lethal dose of the anesthetic solution to allow the removal of the encephala.

Histopathological analysis

After the removal, the encephala were fixed in 10% buffered formaldehyde for 24 hours. The encephala were analyzed and their macroscopic characteristics photographically documented. The macroscopic analysis considered the presence of parenchymal or extra-parenchymal cysticerci, edema, hyperemia, tissue consistency and ventriculomegaly.

Afterwards the encephala were histologically processed, in which the encephala fragments were included in paraffin. After a 5 μm width microtomy the fragments were stained with hematoxilin-eosin[6].

Analysis of the general pathologic processes and classification of the cysticerci into evolutionary stages

The general pathologic processes were evaluated within the parasite, in the host-parasite interface and in the host tissue as to: presence of cysticercus, anatomic location, evolutionary stage of the cysticercus (initial - no buddings, translucent membrane and vesicular fluid; larval - presence of buddings, translucent membrane and vesicular fluid; final - no buddings, opaque membrane and vesicular fluid), ventriculomegaly, parenchymatous and perivascular edema, gliosis, meningitis, ependymitis, choroiditis, perivasculitis, microgliosis, and the profile of inflammatory infiltration cells (polymorphonuclear or mononuclear cells). The pathologic processes detected were classified in a semi-quantitative form according to the following criteria: absent (score 0), discrete - with up to 25% of area commitment (score 1), moderate - 26% to 50% of area commitment (score 2), and accentuated - more than 50% of area commitment (score 3)[6]. The score attributed to each analysis was counted and used to calculate mean ± standard deviation and the statistical analysis.

Statistical analysis

The statistical analysis was performed using the Sigma Stat 2.3 software. All variables were tested for normal distribution and homogenous variation. If they presented non-normal distribution, the variables were analyzed by the nonparametric Mann-Whitney test. The differences were considered significant when p < 0.05.

RESULTS

Macroscopic alterations

The macroscopic alterations were more evident after 30 DPI in BALB/c WT animals, which presented with hyperemia, and decreased encephalic consistency accompanied by intraventricular cysticerci. Most of these animals presented with ventriculomegaly, adjacent parenchymal hypotrophy and/or deviation of the medial line structures ([Table 1], [Figure 1]).

Table 1 Macroscopic analysis of encephala from BALB/c WT and BALB/c IL-4-KO mice experimentally infected with Taenia crassiceps cysticerci.

Variables	07 DPI		30 DPI		60 DPI		90 DPI
	WT	IL-4-KO	WT	IL-4-KO	WT	IL-4-KO	WT	IL-4-KO
Location of cysticerci	LV	LV/3rd V	LV	LV	LV	LV	LV	LV
Edema	+	++	+	+	+	+	+	+
Ventriculomegaly	+	+	+	++	++	++	+++	+++

WT: Wild type; IL-4-KO: IL-4 knockout gene; DPI: days post infection; -: absent; +: discrete; ++: moderate; +++: accentuated; LV: lateral ventricle; 3rd V: third ventricle; n = 5 per each experimental day.

Figure 1 Mesoscopy of BALB/c WT (A) and IL-4-KO BALB/c (B) encephala showing ventriculomegaly and deviation of the medial line. It is possible to observe a cysticercus (arrow) inside the ventricle (scale bar = 1mm).

Cysticerci location and classification

When analyzing all the infected BALB/c animals, it was possible to visualize the cysticerci in 60% (n = 24). In BALB/c WT animals these occurred in 20% (n = 4) of the infected animals, while in BALB/c IL-4-KO infected animals the rate of cysticerci observation was 100% (n = 20). Also, 25% (n = 5) of BALB/c IL-4-KO infected animals showed more than one cysticercus in the encephala.

Regarding the cysticerci evolutionary stage classification, it was possible to observe initial stage parasites at seven DPI, in both mice lineages. At 30 DPI, BALB/c WT animals showed initial stage cysticerci while BALB/c IL-4-KO animals showed larval stage ones. At 60 DPI, both lineages showed larval stage cysticerci. Finally, at 90 DPI, BALB/c WT animals showed final stage cysticerci, while in BALB/c IL-4-KO animals both larval and final stage cysticerci were observed.

As to location, most of the parasites were observed in the lateral ventricles ([Table 1]).

Host tissue response

At seven DPI, the BALB/c IL-4-KO animals showed increased parenchymal inflammatory infiltration of mononuclear cells surrounding the parasite ([Table 2], Figures 2A, 2B), gliosis ([Figure 2C]) and meningitis when compared to BALB/c WT animals (p < 0.05). While the BALB/c WT animals showed an intraventricular cysticercus with inflammatory infiltration of mononuclear cells in the host tissue ([Figure 2D]).

Table 2 Histopathologic analysis of host tissue from encephala of BALB/c WT and BALB/c IL-4-KO mice infected with Taenia crassiceps cysticerci. Results in mean ± standard deviation.

Microscopic alterations	DPI	BALB/c WT	BALB/c IL-4-KO	p
PMN cells inflammatory infiltration	7	1.6 ± 0.89	1.0 ± 0.0	p > 0.05
	30	1.8 ± 0.44	1.6 ± 0.89	p > 0.05
	60	2.6 ± 0.54	1.2 ± 0.83	p =0.014*
	90	1.2 ± 0.44	1.6 ± 0.54	p > 0.05
MN cells inflammatory infiltration	7	1.0 ± 0.0	1.8 ± 0.44	p = 0.032*
	30	1.4 ± 0.54	1.2 ± 0.44	p > 0.05
	60	1.6 ± 0.54	1.2 ± 0.83	p > 0.05
	90	1.4 ± 0.54	1.6 ± 0.54	p > 0.05
Perivasculitis	7	1.6 ± 0.54	0.6 ± 0.89	p > 0.05
	30	1.6 ± 0.54	1.6 ± 0.54	p > 0.05
	60	1.8 ± 0.83	1.2 ± 0.83	p > 0.05
	90	1.0 ± 0.70	2.4 ± 0.54	p > 0.05
Hyperemia	7	1.6 ± 0.54	1.6 ± 0.54	p > 0.05
	30	1.6 ± 0.54	1.4 ± 0.54	p > 0.05
	60	2.4 ± 0.54	1.2 ± 0.44	P = 0.016*
	90	1.2 ± 0.44	2.2 ± 0.44	P = 0.032*
Gliosis	7	0.0 ± 0.0	1.4 ± 0.54	P = 0.008*
	30	2.4 ± 0.89	1.6 ± 0.89	p > 0.05
	60	0.6 ± 0.89	1.2 ± 1.30	p > 0.05
	90	0.8 ± 0.44	1.2 ± 0.44	p > 0.05
Meningitis	7	0.2 ± 0.44	1.4 ± 0.54	P = 0.032*
	30	1.4 ± 0.54	1.6 ± 0.89	p > 0.05
	60	2.4 ± 0.89	1.2 ± 0.83	p > 0.05
	90	0.4 ± 0.54	1.8 ± 0.83	P = 0.014*
Ependymitis	7	1.2 ± 0.44	1.0 ± 0.0	p > 0.05
	30	0.8 ± 0.44	1.2 ± 0.44	p > 0.05
	60	1.0 ± 0.0	0.8 ± 0.44	p > 0.05
	90	1.0 ± 0.70	1.0 ± 0.0	p > 0.05

DPI: days post inoculation; PMN: polymorphonuclear cells; MN: mononuclear cells; BALB/c: BALB mice; WT: wild type; IL-4-KO: IL-4 – Knockout;*statistical difference.

Figure 2 Microscopic alterations in encephala of intracranial infected BALB/c WT and BALB/c IL-4-KO mice. (A-F) hematoxilin-eosin stain, scale bar = 20μm. (A) parenchymal mononuclear cells inflammatory infiltration at seven days post-inoculation (DPI) in BALB/c IL-4-KO; (B) Intraventricular viable cysticercus (asterisk) and mononuclear cell inflammatory infiltration (arrow) in the host tissue at seven DPI in BALB/c WT; (C) Gliosis, at seven DPI in BALB/c IL-4-KO; (D) meningitis at 90 DPI in BALB/c IL-4-KO; (E) intraventricular polymorphonuclear cell inflammatory infiltration (asterisk) at 60 DPI in BALB/c WT; (F) hyperemia and perivascular edema (arrow) at 90 DPI in BALB/c IL-4-KO.

At 60 DPI, BALB/c WT animals showed higher inflammatory infiltration of polymorphonuclear cells and hyperemia when compared to BALB/c IL-4-KO animals (p < 0.05). In BALB/c WT animals, it was possible to observe an inflammatory infiltration of mononuclear cells inside the ventricles ([Figure 2E]).

At 90 DPI, BALB/c IL-4-KO animals showed higher hyperemia and meningitis when compared to BALB/c WT animals (p < 0.05) ([Figure 2F]).

The control group animals from both lineages showed hyperemia and edema in discrete intensity in the first days after the inoculation of saline sterile solution.

DISCUSSION

Helminths such as T. crassiceps cysticerci have developed complex mechanisms to evade or modulate the host response during infection. This study describes the influence of an important cytokine such as IL-4 in response to helminthic infection. The use of genetically modified mice lineages is an important tool in the understanding of the host-parasite relationship to the development of the parasitic infection and, consequently, the clinical manifestations of the infection[7].

All the infected animals showed both micro- and macroscopic alterations, even when the cysticerci were not visible on macroscopic analysis. All the control animals showed discrete levels of edema and hyperemia due to the mechanical trauma of the inoculation of sterile saline solution. These discrete alterations in the control group have also been observed in other experimental NCC studies[6],[13],[18],[21].

In the infected animals, the majority of the cysticerci were located inside the lateral ventricles accompanied by a discrete-to-moderate intensity of inflammatory response. This fact was previously reported in studies that showed that the mere presence of the parasite’s antigens are responsible for the development of inflammatory alterations and ventriculomegaly, which may lead to cerebrospinal fluid obstruction[22],[23]. Zepeda et al.[21] showed that T. crassiceps antigens are able to induce inflammation in the brain tissue and apoptosis of hippocampal cells. Histological studies have shown that viable cysticerci, such as the ones in our study, have little or no surrounding inflammation in humans and pigs; while degenerating or dead cysticerci, which were not found in our study, had an inflammatory response in their surroundings[24].

In our study, all infected animals showed ependymitis, meningitis and perivasculitis. These alterations have also been described in experimental NCC in rats[19]. In our study, it was not possible to observe calcification of the cysticerci, or in the surrounding host tissue. However, the calcification of the cysticerci and host tissue was observed in autopsies of human intraventricular NCC[25] and in a swine model of NCC[2], probably because the material analyzed had a longer time of infection.

The initial stage cysticerci showed a discrete intensity of inflammatory infiltration surrounding the parasite. As the cysticerci die, the immune modulation is lost, resulting in an increase in the inflammatory intensity accompanied by fibrosis. Alvarez et al.,[5] studying experimental NCC, reported that the parasite initiates the immunomodulation through complex glycoproteins present in the tegument surface resulting in the viability of the parasite. The histopathologic analysis of human asymptomatic NCC shows viable cysticerci surrounded by discrete or absent inflammatory infiltration that contributes to the chronicity of the infection[25]. On the other hand, Fleury et al.[25], studying cysticerci removed from human NCC, reported that the parasite is surrounded by human immunoglobulin such as IgG, IgM, IgA and IgE, which act as a kind of camouflage due to the presence of Fc receptors in the parasite tegument surface.

In BALB/c WT animals it was possible to observe that the peak evolution of the encephalitis was accompanied by a partial destruction of the cysticerci in the experimental days. In the BALB/c IL-4-KO animals, the evolution of the inflammatory process was not resolved due to the presence of viable cysticerci until the end of the experimental days. The absence of IL-4 resulted in difficulty in controlling the parasitic infection. A Th1 immune response is observed while the parasite is still viable, while a mixed Th1/Th2 immune response is observed when the parasite is being destroyed[26]. Experimental NCC studies have shown an inflammatory infiltration of macrophages and plasmocytes in the high levels of tissue IL-4[25] in rats and pigs. These findings show that the parasite maintains an equilibrium with the host immune response at the beginning of the infection and therefore a rapid Th1 response is observed, leading to the production of IgG. However, as the infection progresses the balance is modified to a mixed Th1/Th2 response, which may result in destruction of the cysticerci[27].

When mononuclear cells from human asymptomatic NCC patients are stimulated in vitro, it is possible to observe the increased production of IL-4, IL-5 and IL-13, which are Th2 cytokines[27]. However, when the mononuclear cells are collected from symptomatic NCC patients, the profile of the cytokines produced are quite different, with a predominance of IFN-gamma, IL-17, IL-23 and TNF-alpha, which are from the Th2 profile[24]. Asymptomatic NCC patients from endemic areas have shown a Th2 response with production of IgG4, IL-4, IL-5 and IL-13[27].

In the M. corti experimental NCC model, it was observed that the Th1 immune profile was related to a strong immune response capable of destruction of the parasite and tissue injury[13]. Matos-Silva et al.[6] also found similar results using the T. crassiceps NCC model. Using the same model, Moura et al.[18] described a predominance of the Th2 immune profile cytokines at the end of the infection, while in the acute phase of the infection there was a mixed Th1/Th17 profile accompanied by high levels of IL-10.

BALB/c animals are naturally susceptible to T. crassiceps infection, both in the intraperitoneal and intracranial locations, due to its natural development of a Th2 profile response[7],[28]. The main cytokine of the Th2 profile is IL-4 as it induces activation and effectiveness of T lymphocytes and also stimulates the change of lymphocyte B isotype into IgE production. As well, IL-4 suppresses the IFN-gamma activity of macrophages resulting in the inhibition of these cells[7],[26].

Therefore an immune Th2 response, which is considered protective against intestinal helminthic infections, is harmful to the host when it favors the establishment and growth of tissue helminthes[26]. Greater parasitic burden was observed in Th2 immune profile animals studied by Rodrigues-Sosa et al.[26], as well as in our findings in which we observed cysticerci in all BALB/c IL-4-KO infected animals.

In central nervous system infections, IL-4 plays an important role in the immune regulation due to stimulation and chemotaxis of eosinophils, mastocytes, basophils and other Th2 profile cells. Also, the survival of these cells are maintained by the influence of IL-4[29]. Experimental studies have shown the influence of this cytokine as a neuroprotective factor on astrocytes and microglia[29],[30]. Falcone et al.[30], studying an experimental model of autoimmune encephalitis in IL-4-KO mice, reported that the animals without IL-4 showed a more severe form of clinical disease, with extensive involvement of spinal cord accompanied by increased production of pro-inflammatory cytokines.

According to the anatomopathological findings and the analysis of the inflammatory process in the infected animals, it is possible to conclude that the absence of IL-4 induced less intense inflammatory alterations. This response probably retarded the destruction of the cysticerci, leading to a greater parasitic burden, tissue destruction and chronicity of the infection.

Conflict of interest:

There is no conflict of interest to declare.

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Publikationsverlauf

Eingereicht: 02. Mai 2016

Angenommen: 31. Oktober 2016

Artikel online veröffentlicht:
05. September 2023