Assessment of canine autologous platelet-rich plasma produced with a commercial centrifugation and platelet recovery kit

Chris W. Frye; Andrew Enders; Marjory B. Brooks; Angela M. Struble; Joseph J. Wakshlag

doi:10.3415/VCOT-15-03-0046

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2016; 29(01): 14-19
DOI: 10.3415/VCOT-15-03-0046

Original Research

Schattauer GmbH

Assessment of canine autologous platelet-rich plasma produced with a commercial centrifugation and platelet recovery kit

Authors

Chris W. Frye

¹Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
Andrew Enders

¹Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
Marjory B. Brooks

²Department of Population Medicine, Cornell University College of Veterinary Medicine, Ithaca, NY USA
Angela M. Struble

¹Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA
Joseph J. Wakshlag

¹Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY, USA

Abstract

Summary

Objectives: To characterize the cellular composition (platelets, erythrocytes, and leukocytes) and confirm reproducibility of platelet enrichment, as well as determine the platelet activation status in the final product of a commercial platelet-rich plasma kit using canine blood.

Methods: Venous blood from 20 sedated client-owned dogs was used to prepare platelet-rich plasma (PRP) from a commercial kit. Complete blood counts were performed to determine erythrocyte, leukocyte, and platelet numbers in both whole blood (WB) and resultant PRP. The WB and PRP samples from jugular (fast collection) and cephalic (slow collection) venipuncture were also compared. P-selectin externalization was measured in WB and PRP samples from 15 of 20 dogs.

Results: This commercial kit produced an average percent recovery in platelets of 64.7 ± 17.4; erythrocytes of 3.7 ± 0.8, and leukocytes of 31.6 ± 10.0. Neutrophil, monocyte, and lymphocyte percent recovery was 19.6 ± 7.2, 44.89 ± 19.8, and 57.5 ± 10.6, respectively. The recovery of platelets from jugular venipuncture (59.7 ± 13.6%) was lower than from cephalic recovery (68.8 ± 19.1%). The mean percent P-Selectin externalization for WB, PRP, and PRP with thrombin was 25.5 ± 30.9, 4.5 ± 6.4, and 90.6 ± 4.4 respectively.

Clinical significance: Cellular reproducibility of this kit was confirmed and platelets were concentrated within autologous serum. Additionally, measurements of P-selectin externalization showed that platelets are inactive in PRP unless stimulated to degranulate.

Keywords

Platelet-rich plasma - PRP - P-selectin - platelet activation - leukocytes - canine

Full Text

References

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