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Horm Metab Res 1975; 7(1): 88-92
DOI: 10.1055/s-0028-1093757
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© Georg Thieme Verlag KG Stuttgart · New York

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland[*]

P. R. Oeltgen [**] , M. V. L'Heureux
  • Department of Biochemistry and Biophysics, Stritch School of Medicine and the Graduate School, Loyola University of Chicago, Maywood, Illinois, USA
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Publication Date:
23 December 2008 (online)

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Abstract

Mitochondria, nuclei, and microsomes obtained from ovine thyroid slices which had been incubated in Krebs-Ringer bicarbonate buffer containing 4.8, 11.5 or 26.0 mg% concentration of calcium were assayed for calcitonin activity in rats. The microsomal preparation was the only particulate fraction to exhibit marked hypocalcemic activity and the hormonal activity of this fraction was enhanced by a 26.0 mg% calcium concentration in the incubation medium. The identity of the hypocalcemic species in the microsomal fraction with a calcitonin preparation of known potency was established by Polyacrylamide gel eletrophoresis and by isoelectric focusing. The ovine microsomal calcitonin was resolved into two protein bands with isoelectric points (pl's) of 3.49 and 3.84. Both of these protein peaks exhibited hypocalcemic activity. The porcine standard calcitonin was resolved into two protein bands with pl's of 3.70 and 3.94.

Key words

Ovine - Calcium - Calcitonin - Biosynthesis - Particulate Fractions - Isoelectric Point

1 This work was supported in part by USPHS, Training Grant 512GM698-06.

1 This work was supported in part by USPHS, Training Grant 512GM698-06.

2 The data in this paper are taken from a dissertation submitted by Peter R. Oeltgen in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Loyola University of Chicago, February, 1973.
Present address: Department of Biochemistry, Loyola University School of Dentistry, Maywood, Illinois 60153, U.S.A. Present address: Department of Biochemistry, Loyola University School of Dentistry, Maywood, Illinois 60153, U.S.A.