Planta Med 2009; 75(14): 1470-1475
DOI: 10.1055/s-0029-1185802
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Astilbin on Experimental Diabetic Nephropathy in vivo and in vitro

Gui-Sheng Li1 [*] , Wang-Lin Jiang1 , 2 [*] , Xi-Dian Yue2 , Gui-Wu Qu2 , Jing-Wei Tian1 , Juan Wu2 , Feng-Hua Fu1
  • 1School of Pharmacy, Yantai University, Yantai, P. R. China
  • 2Shandong Engineering Research Center for Natural Drug, Yantai, P. R. China
Further Information

Publication History

received March 2, 2009 revised May 7, 2009

accepted May 17, 2009

Publication Date:
16 June 2009 (online)

Abstract

Astilbin, a flavonoid compound, was isolated from the rhizome of Smilax glabra Roxb. This study was conducted to investigate the efficacy of astilbin on experimental diabetic nephropathy (DN) in vivo and in vitro and its possible mechanisms. Astilbin was added in high glucose stimulated HK-2 cells, streptozotocin-induced experimental DN, randomized to receive intragastric (i. g.) astilbin to observe its anti-renal lesion effect. Results showed that astilbin inhibited high glucose stimulated HK-2 cell production of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in vitro, especially CTGF; analogic results was also found in vivo. I. g. of astilbin 2.5 mg/kg or 5 mg/kg significantly ameliorated renal function, reduced kidney index, while it increased body weight and survival time in animals. In addition there was no significant difference in blood glucose level between the STZ-treated group and the astilbin groups. Furthermore, astilbin ameliorated the pathological progress of renal morphology. Astilbin can exert an early renal protective role to DN, inhibit production of TGF-β1 and especially of CTGF. We suggest that astilbin inhibition of CTGF may be a potential target in DN therapy. This work provides the first evidence for astilbin as a new candidate of DN therapeutic medicine.

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1 These authors contributed equally to this project and should be considered co-first authors.

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