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Planta Med 2012; 78(09): 926-933
DOI: 10.1055/s-0031-1298496
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Metabolic Fingerprinting by 1HNMR for Discrimination of the Two Species Used as Radix Bupleuri

Xuemei Qin
1   Center of Modern Research for Traditional Chinese Medicine, Shanxi University, Taiyuan, Peopleʼs Republic of China
2   Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
,
Yuntao Dai
2   Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
,
Ning Qing Liu
2   Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
,
Zhenyu Li
1   Center of Modern Research for Traditional Chinese Medicine, Shanxi University, Taiyuan, Peopleʼs Republic of China
,
Xiaojie Liu
1   Center of Modern Research for Traditional Chinese Medicine, Shanxi University, Taiyuan, Peopleʼs Republic of China
,
Jie Hu
1   Center of Modern Research for Traditional Chinese Medicine, Shanxi University, Taiyuan, Peopleʼs Republic of China
,
Young Hae Choi
2   Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
,
Robert Verpoorte
2   Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

received 17 February 2012
revised 26 March 2012

accepted 04 April 2012

Publication Date:
26 April 2012 (online)

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Abstract

Radix Bupleuri is a traditional Chinese medicine harvested from two Bupleurum species (B. chinense and B. scorzonerifolium). It is widely used and is sourced from different regions of China. 1H NMR spectroscopy and multivariate data analysis were applied to 67 Radix Bupleuri samples to discriminate the two species, and explore the influences of habitat and culture method on the quality of Radix Bupleuri based on their metabolomics profiles. Metabolites responsible for the differences between the two species were higher levels of arginine, citric acid, sucrose, saikosaponin b1/b2 analogs, volatile oil with an (E)-2-olefin aldehyde fragment, and fatty acids in B. scoreonerifolium, and more saikosaponin a/c/d analogs in B. chinense. The variances of two cultivation areas were observed due to the higher amount of saikosaponins a/c/d in samples from Shaanxi and lipidsin samples from Shanxi. No obvious difference was detected between cultivars and wild type. 1HNMR metabolomics can simultaneously detect saikosaponins and hydrocarbon aldehydes, and also differentiate the two main saikosaponin skeletons, making it a suitable tool for the species discrimination and quality evaluation of Radix Bupleuri.

Key words

metabolomics - 1H-NMR - Apiaceae - Radix Bupleuri - Bupleurum chinense - Bupleurum scorzonerifolium - saikosaponins - quality control
 

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