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Drug Res (Stuttg) 2013; 63(09): 450-456
DOI: 10.1055/s-0033-1343471
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Development and Validation of a Reverse Phase Liquid Chromatography Method for the Simultaneous Quantification of Eserine and Pralidoxime Chloride in Drugs-in-Adhesive Matrix Type Transdermal Patches

S. Banerjee
1   Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam, India
2   Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India
,
P. Chattopadhyay
1   Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam, India
,
A. Ghosh
2   Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India
,
S. Kaity
2   Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India
,
V. Veer
1   Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, Assam, India
› Author Affiliations
Further Information

Publication History

received 14 January 2013

accepted 29 March 2013

Publication Date:
30 April 2013 (online)

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Abstract

Background:

In the present study, a simple, precise, specific, fast, accurate and reliable reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous determination and quantification of eserine and pralidoxime chloride in drugs-in-adhesive matrix type transdermal patches.

Methods:

The chromatographic separation was achieved by C18 column, using a mobile phase consisting of acetonitrile: 10 mM potassium dihydrogen phosphate, 10 mM heptane-1-sulfonic acid sodium salt monohydrate in water (30:70, v/v) adjusted at pH 3.0 with ortho-phosphoric acid. Flow rate was 1.0 mL/min and UV detection at 238 nm. The method was validated according to the International Conference on Harmonization (ICH) guidelines.

Results:

The calibration curves were linear over the different concentration ranges of 0.5–10 μg/ml for eserine and 5–25 μg/mL for 2PAM. Relative standard deviation for precision was less than 2.0%. Limit of detection values of eserine and 2-PAM were 0.018 µg/mL and 0.008 µg/mL, respectively. The limit of quantification of eserine and 2-PAM were 0.055 µg/mL and 0.026 µg/mL, respectively.

Conclusion:

The developed method was applied for the routine analysis of these 2 drugs in drugs-in-adhesive matrix type transdermal patches in order to evaluate the drug content of different formulations. It could be also used with reliability for the determination of the drug in other pharmaceutical dosage forms.

Key words

validation - HPLC - simultaneous determination - eserine - pralidoxime chloride - transdermal patch
 

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