Subscribe to RSS
DOI: 10.1055/s-0038-1646490
Improved Adhesion and Proliferation of Human Endothelial Cells on Polyethylene Precoated with Monoclonal Antibodies Directed against Cell Membrane Antigens and Extracellular Matrix Proteins
Publication History
Received 29 January 1991
Accepted 06 June 1991
Publication Date:
26 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199106/lookinside/thumbnails/10-1055-s-0038-1646490-1.jpg)
Summary
Endothelial cell seeding may improve the patency of synthetic vascular grafts provided that platelet reactivity of non-endothelialized sites is not increased. We have investigated if surface-adsorbed monoclonal antibodies directed against endothelial cell membrane proteins and against extracellular matrix proteins promote the adhesion and proliferation of cultured human endothelial cells, without causing platelet deposition at non-endothelialized sites. Adhesion of endothelial cells onto polyethylene coated with monoclonal antibodies directed against endothelial cell-specific membrane antigens, integrin receptors and glycoprotein CD31 was equal to or higher than adhesion onto fibronectin-coated polyethylene. Endothelial cells did not proliferate on these surface-adsorbed antibodies. However, pre-coating of polyethylene with mixtures of endothelial cell-specific monoclonal antibodies and monoclonal antibodies directed against fibronectin or von Willebrand factor, resulted in relatively high adhesion and optimal proliferation. Platelet reactivity of the polyethylene surface was found to significantly increase after adsorption of fibronectin, endothelial cell-specific monoclonal antibody or its Fc fragments. In contrast, adsorption of F(ab')2 fragments of endothelial cell-specific monoclonal antibody did not promote platelet deposition. Therefore, it is concluded that coating of vascular graft materials with mixtures of F(ab')2 fragments of monoclonal antibodies specifically directed against endothelial cells and against extracellular matrix proteins may be an effective way to both promote the growth of seeded endothelial cells and limit platelet-graft interaction.
-
References
- 1 Snyder RW, Botzko KM. Woven, knitted and externally supported Dacron vascular prostheses. In: Biological and Synthetic Vascular Prostheses.. Stanley JC, Burkel WE, Lindenauer SM, Bartlett RH, Turcotte JG. (eds) Grune and Stratton, New York: 1982. p 485
- 2 Graham LM, Bergan JJ. Expanded polytetrafluoroethylene vascular grafts: clinical and experimental observations. In: Biological and Synthetic Vascular Prostheses.. Stanley JC, Burkel WE, Lindenauer SM, Bartlett RH, Turcotte JG. (eds) Grune and Stratton, New York: 1982. p 563
- 3 Jarrell BE, Williams SK, Solomon L, Speicher L, Koolpe E, Radomski J, Carabasi RA, Greener D, Rosato FE. Use of an endothelial monolayer on a vascular graft prior to implantation. Temporal dynamics and compatibility with the operating room. Ann Surg 1986; 203: 671
- 4 Van Wachem PB, Beugeling T, Feijen J, Bantjes A, Detmers JP, van Aken WG. Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. Biomaterials 1985; 6: 403
- 5 Herring MB. Endothelial seeding of blood flow surfaces. In: Vascular Grafting, Clinical Applications and Techniques.. Wright CB. (ed) John Wright PSG Inc., Boston: 1983. p 275
- 6 Wu KK, Frasier-Scott K, Hatzakis H. Endothelial cell function in hemostasis and thrombosis. Adv Exp Med Biol 1988; 242: 127
- 7 Stanley JC, Burkel WE, Graham LM, Lindblad B. Endothelial cell seeding of synthetic vascular prostheses. Acta Chir Scand Suppl 1985; 529: 17
- 8 Örtenwall P, Wadenvik H, Kutti J, Risberg B. Reduction in deposition of Indium 111-labeled platelets after autologous endothelial cell seeding of Dacron aortic bifurcation grafts in humans: a preliminary report. J Vasc Surg 1987; 6: 17
- 9 Dekker A, Reitsma K, Beugeling T, Bantjes A, Feijen J, van Aken WG. Adhesion of cultured human endothelial cells and adsorption of serum proteins on gas plasma treated PTFE films and ePTFE graft material in relation to the surface characteristics. Biomaterials,. in press
- 10 Graham LM, Stanley JC, Burkel WE. Improved patency of endothelial cell seeded, long, knitted Dacron and ePTFE vascular prostheses. ASAIO J 1985; 8: 65
- 11 Seeger JM, Klingman N. Improved in vivo endothelialization of prosthetic grafts by surface modification with fibronectin. J Vasc Surg 1988; 8: 476
- 12 Allen BT, Long JA, Clark RE, Sicard GA, Hopkins KT, Welch MJ. Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts. J Vasc Surg 1984; 1: 224
- 13 Dekker A, Poot A, Beugeling T, Bantjes A, van WG. The effect of vascular cell seeding on platelet deposition in an in vitro capillary perfusion model. Thromb Haemostas 1989; 61: 402-8
- 14 Aplin JD, Hughes RC. Cell adhesion on model substrata: threshold effects and receptor modulation. J Cell Sci 1981; 50: 89
- 15 Grinnell F, Hays DG. Induction of cell spreading by substratum-adsorbed ligands directed against the cell surface. Exp Cell Res 1978; 116: 275
- 16 Poot A, Beugeling T, Cazenave JP, Bantjes A, van Aken WG. Platelet deposition in a capillary perfusion model: quantitative and morphological aspects. Biomaterials 1988; 9: 126
- 17 Van Mourik JA, Leeksma OC, Reinders JH, de Groor PhG, Zandbergen-Spaargaren J. Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa. J Biol Chem 1985; 260: 11300
- 18 Sbarbati R, de Boer M, Marzilli M, Scarlattini M, Rossi G, van Mourik JA. Immunological detection of endothelial cells in human whole blood. Blood, in press
- 19 Reinders JH. Endothelial cell von Willebrand factor. PhD Thesis, University of Amsterdam, Amsterdam: 1986. p 63
- 20 Giltay JC, Brinkman HJM, Modderman PW, von dem Borne AEGK, van Mourik JA. Human vascular endothelial cells express a membrane protein complex immunochemically indistinguishable from the platelet VLA-2 (glycoprotein Ia - IIa) complex. Blood 1989; 73: 1235
- 21 Tetteroo AT, Lansdorp PM, Leeksma OC, von dem Borne AEGK. Monoclonal antibodies against human platelet glycoprotein IIIa. Br J Haematol 1983; 55: 509
- 22 Modderman PW, Huisman HG, van Mourik JA, von dem Borne AEGK. A monoclonal antibody to the human platelet glycoprotein IIb/IIIa complex induces platelet activation. Thromb Haemostas 1988; 60: 68-74
- 23 Leeksma OC, Zandbergen-Spaargaren J, Giltay JC, van Mourik JA. Cultured human endothelial cells synthesize a plasma membrane protein complex immunologically related to the platelet glycoprotein IIb/IIIa complex. Blood 1986; 67: 1176
- 24 Newman PJ, Berndt MC, Gorski J, White GC, Lyman S, Paddock C, Muller WA. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 1990; 247: 1219
- 25 Stel HV, Sakariassen KS, Scholte BJ, Veerman ECI, van der Kwast TM, de Grpot PhG, Sixma JJ, van Mourik JA. Characterization of 25 monoclonal antibodies to Factor VIII – von Willebrand factor. Relation between ristocetin-induced platelet aggregation and platelet adherence to subendothelium. Blood 1984; 63: 1408
- 26 De Groot PhG, Ottenhof-Rovers M, van Mourik JA, Sixma JJ. Evidence that the primary binding site of von Willebrand factor that mediates platelet adhesion on subendothelium is not collagen. J Clin Invest 1988; 82: 65
- 27 Reinders JH, de Groot PhG, Dawes J, Hunter NR, van Heugten HAA, Zandbergen J, Gonsalves MD, van Mourik JA. Comparison of secretion and subcellular localization of von Willebrand protein with that of thrombospondin and fibronectin in cultured human vascular endothelial cells. Biochim Biophys Acta 1985; 844: 306
- 28 Poot A, Beugeling T, van Aken WG, Bantjes A. Detection of surface-adsorbed (lipo)proteins by means of a two-step enzyme-immunoassay: a study of the Vroman effect. J Biomed Mater Res 1990; 24: 1021
- 29 Heuvelsland WJM, Dubbeldam GC, Brouwer W. Reflectometry as a tool for dynamically measuring protein adsorption. In: Test Procedures for the Blood Compatibility of Biomaterials.. Dawids S. (ed) to be published
- 30 Willems Ch, Astaldi GCB, de Groot PhG, Jansen MC, Gonsalves MD, Zeijlemaker WP, van Mourik JA, van Aken WG. Media conditioned by cultured vascular endothelial cells inhibit the growth of vascular smooth muscle cells. Exp Cell Res 1982; 139: 191
- 31 Van Wachem PB, Reinders JH, van Buul-Wortelboer MF, de Groot PhG, van Aken WG, van Mourik JA. Von Willebrand factor in cultured human vascular endothelial cells from adult and umbilical cord arteries and veins. Thromb Haemostas 1986; 56: 189-92
- 32 West DC, Sattar A, Kumar S. A simplified in situ solubilization procedure for the determination of DNA and cell number in tissue cultured mammalian cells. Anal Biochem 1985; 147: 289
- 33 Mates G, Daniel M, Walker C. Factors affecting the reproducibility of a spectrofluorometric assay for the enumeration of human venous endothelium in culture. Cell Biol Int Rep 1986; 10: 641
- 34 Van Wachem PB, Mallens BWL, Dekker A, Beugeling T, Feijen J, Bantjes A, Detmers JP, van Aken WG. Adsorption of fibronectin derived from serum and from human endothelial cells onto tissue culture polystyrene. J Biomed Mat Res 1987; 21: 1317
- 35 Ruoslahti E, Pierschbacher MD. New perspectives in cell adhesion: RGD and integrins. Science 1987; 238: 491
- 36 Ginsberg MH, Loftus JC, Plow EF. Cytoadhesins, integrins and platelets. Thromb Haemostas 1988; 59: 1-6
- 37 Hynes RO. Integrins: a family of cell surface receptors. Cell 1987; 48: 549
- 38 Reinders JH, de Groot PhG, Gonsalves MD, Zandbergen J, Loesberg C, van Mourik JA. Isolation of a storage and secretory organelle containing von Willebrand protein from cultured human endothelial cells. Biochim Biophys Acta 1984; 804: 361
- 39 Yamada KM, Olden K. Fibronectins: adhesive glycoproteins of cell surface and blood. Nature 1978; 275: 179
- 40 Bornstein P, Ash JF. Cell surface-associated structural proteins in connective tissue cells. Proc Natl Acad Sci USA 1977; 74: 2480
- 41 Pearlstein E, Gold LI, Garcia-Pardo A. Fibronectin: a review of its structure and biological activity. Molec Cellul Biochem 1980; 29: 103
- 42 Van Wachem PB, Vreriks CM, Beugeling T, Feijen J, Bantjes A, Detmers JP, van Aken WG. The influence of protein adsorption on interactions of cultured endothelial cells with polymers. J Biomed Mater Res 1987; 21: 701
- 43 Schwarz MA, Brown PJ, Eveleth DD, Bradshaw RA. Modulation of growth factor-induced fiber outgrowth in rat pheochromocytoma (PC12) cells by a fibronectin receptor antibody. J Cell Physiol 1989; 138: 121
- 44 Werb Z, Tremble PM, Behrendtsen O, Crowley E, Damsky CH. Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression. J Cell Biol 1989; 109: 877
- 45 Rubini M, Panozzo M, Selvatici R, Baricordi OR, Mazzilli MC, Pozzan T, Gandini E. An anti-HLA class I monoclonal antibody alters the progression in the cell cycle of phytohemagglutinin-activated human T lymphocytes. Exp Cell Res 1990; 187: 11
- 46 Ingber DE, Folkman J. Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix. J Cell Biol 1989; 109: 317
- 47 Ingber DE. Fibronectin controls capillary endothelial cell growth by modulating cell shape. Proc Natl Acad Sci USA 1990; 87: 3579
- 48 Dekker A, Beugeling T, Wind H, Poot A, Bantjes A, Feijen J, van Aken WG. Deposition of cellular fibronectin and desorption of human serum albumin during adhesion and spreading of human endothelial cells on tissue culture polystyrene. In preparation
- 49 Feijen J, Beugeling T, Bantjes A, Smit Sibinga CTh. Biomaterials and interfacial phenomena. In: Advances in Cardiovascular Physics 3.. Ghista DN. (ed) S Karger, Basel: 1979. p 100
- 50 Harrison DD, Culp LA. Adhesive responses of fibroblast and neuroblastoma cells to substrata coated with polyvalent or monoclonal antibody to fibronectin. Exp Cell Res 1983; 146: 29
- 51 Henson PM, Spiegelberg HL. Release of serotonin from human platelets induced by aggregated immunoglobulins of different classes and subclasses. J Clin Invest 1973; 52: 1282