Download PDF
Thromb Haemost 1976; 35(02): 305-313
DOI: 10.1055/s-0038-1647923
Original Article
Schattauer GmbH

Fibrinogen and its Derivatives: Cofactors in the Intrinsic Generation of Thrombin

D.C Triantaphyllopoulos
1   American National Red Cross, Blood Research Laboratory, Bethesda, Maryland 20014 and Department of Physiology and Biophysics, Georgetown University School of Medicine, Washington, D. C. 20007
,
L.T Ryan
1   American National Red Cross, Blood Research Laboratory, Bethesda, Maryland 20014 and Department of Physiology and Biophysics, Georgetown University School of Medicine, Washington, D. C. 20007
› Author Affiliations
Further Information

Publication History

Received 10 July 1975

Accepted 02 November 1975

Publication Date:
02 July 2018 (online)

    Permissions and Reprints

Summary

The simultaneous addition of suboptimal concentrations of factor VIII and intact or plas-min-lysed fibrinogen into mixtures of the vitamin K dependent factors, phospholipids, adsorbed bovine serum (supplier of factor V) and calcium, increased the amount of thrombin which was generated three to twenty times over the sum of the amounts which were generated when factor VIII, or fibrinogen, or its derivatives were added separately into the thrombin generating mixture. When factor VIII was added together with both fibrinogen and its derivatives, the amount of thrombin generated was even greater, about 130% larger than the amount which was generated in the presence of equal concentrations of only intact fibrinogen plus factor VIII. Addition of albumin instead of fibrinogen or its derivatives has a similar but significantly lower effect on thrombin generation. It appears, therefore, that both intact fibrinogen and its plasminolytic derivatives, singly or in combination, and to a lesser extent albumin, act as cofactors in the reaction which is regulated by factor VIII.

 

  • References

  • 1 Aronson D. L, Mustafa A. J, Mushinski J. F. 1969; Purification of human factor X and comparison of peptide maps of human factor X and prothrombin. Biochimica Biophysica Acta 188: 25.
    CrossrefPubMedGoogle Scholar
  • 2 Barrow E. S, Lester R. H, Johnson A. M, Graham J. B. 1972; Production of antihemophilic factor (factor VJII) from albumin. American Journal of Physiology 222: 920.
    PubMedGoogle Scholar
  • 3 Biggs R. (ed.) 1972. Human Blood Coagulation, Haemostasis and Thrombosis. Blackwell Scientific Publications; Oxford: 609.
    Google Scholar
  • 4 Biggs R, MacFarlane R. G, Denson K. W. E, Ash B. T. 1965; Thrombin and the interaction of factors VIII and IX. British Journal of Haematology 11: 276.
    CrossrefPubMedGoogle Scholar
  • 5 Chandra S, Triantaphyllopoulos D. C. 1972; Preparation of degradation products of fibrinogen free of plasmin, and their effect on thrombin activity. Canadian Journal of Physiology and Pharmacology 50: 655.
    CrossrefPubMedGoogle Scholar
  • 6 Chandra S, Triantaphyllopoulos D. C. 1973; Potentiation of the antifactor VIII effect of thrombin in plasma by the plasminolytic derivatives of fibrinogen and fibrin. Thrombosis et Diathesis Haemorrhagica 30: 235.
    PubMedGoogle Scholar
  • 7 Cuatrecasas P, Wilchek M, Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proceedings of the National Academy of Sciences 61. 636.
    PubMedGoogle Scholar
  • 8 Goldstein R, Zonderman E. B. 1958 Purification of Drothrombin and its separation from other coagulation factors. Proceedings of the International Congress, International Society of Haematology, Rome,. II Pensiero Scientifico, p. 1006.
    PubMedGoogle Scholar
  • 9 Kirby E. P, Martin N, Marder V. J. 1974; Degradation of bovine factor VIII by plasmin and trypsin. Blood 43: 629.
    PubMedGoogle Scholar
  • 10 Klein P. D, Seegers W. H. 1950; The nature of plasma antithrombin activity. Blood 5: 742.
    PubMedGoogle Scholar
  • 11 Mysliwiec M, Arnesen H, Godal H. C. 1973; The effect of fibrinogen degradation products on plasmin activity. Thrombosis et Diathesis Haemorrhagicia 29: 592.
    PubMedGoogle Scholar
  • 12 Newman J, Johnson A. J, Karpatkxn M. H, Puszkin S. 1971; Methods for the production of clinically effective intermediate and high purity factor VIII concentrates. British Journal of Haematology 21: 1.
    CrossrefPubMedGoogle Scholar
  • 13 Osterud B, Rapaport S. I, Scheffman S, Chong M. M. Y. 1971; Formation of intrinsic factor X-activator activity with special reference to the role of thrombin. British Journal of Haematology 643: 1971.
    PubMedGoogle Scholar
  • 14 Penick G. D. 1957; Some factors that influence utilization of antihemophilic activity during clotting. Proceedings of the Society of Experimental Biology and Medicine 96: 211.
    PubMedGoogle Scholar
  • 15 Seegers W. H. 1962. Prothrombin. Harvard University Press; Cambridge, Mass: 94.
    Google Scholar
  • 16 Seegers W. H, Landaburu R. H, Fenichel R. L. 1957; Isolation of platelet cofactor I from plasma and some properties of the preparation. American Journal of Physiology 190: 1.
    PubMedGoogle Scholar
  • 17 Sgouris J. T, Inman J. K, McCall K. B, Hyndman L. A, Anderson H. D. 1960; The preparation of human fibrinolysin (plasmin). Vox Sanguinis 5: 1960.
    PubMedGoogle Scholar
  • 18 Sgouris J. T, Wickerhauser MD. 1973; Use of frozen cryoprecipitate for the preparation of clinical factor VIII concentrate. Transfusion 13: 399.
    CrossrefPubMedGoogle Scholar
  • 19 Siegelman A. M, Carlson A. S, Robertson T. 1962; Investigation of serum trypsin and related substances. I. The quantitative demonstration of trypsin-like activity in human blood serum by a micro-method. Archives of Biochemistry 97: 159.
    CrossrefPubMedGoogle Scholar
  • 20 Triantaphyllopoulos D. C. 1958; Anticoagulant effect of incubated fibrinogen. Canadian Journal of Biochemistry and Physiology 36: 249.
    CrossrefPubMedGoogle Scholar
  • 21 Triantaphyllopoulos D. C, Brunetti A. D, Triantaphyllopoulos E. 1970; Increased prothrombin conversion: A new activity of lysed fibrinogen. British Journal of Haematology 18: 127.
    CrossrefPubMedGoogle Scholar
  • 22 Triantyphyllopoulos E, Triantaphyllopoulos D. C. 1965; Variability of the anticoagulant fraction of incubated fibrinogen. American Journal of Physiology 208: 521.
    PubMedGoogle Scholar
  • 23 Triantaphyllopoulos E, Triantaphyllopoulos D. C. 1967; New anticoagulant from lysed fibrinogen. British Journal of Haematology 13: 28.
    CrossrefPubMedGoogle Scholar
  • 24 Triantaphyllopoulos E, Triantaphyllopoulos D. C. 1973; Selected topics in blood coagulation. C. R. C. Critical Reviews in Biochemistry 1: 305.
    PubMedGoogle Scholar
  • 25 Ware A. G, Seegers W. H. 1949; Two stage procedure for the quantitative determination of prothrombin concentration. American Journal of Clinical Pathology 19: 471.
    CrossrefPubMedGoogle Scholar