Thromb Haemost 1967; 17(03/04): 516-531
DOI: 10.1055/s-0038-1654176
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Platelet Aggregation by Magnesium Ion

R. M Des Prez M. D.,
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
R. E Bryant M. D.,
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
J. A Katz
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
Th. E Brittingham M. D.
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
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Supported by research grants HE 08399 and AI 03082 from the National Institutes of Health, U. S. Public Health Service.
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Publikationsdatum:
26. Juni 2018 (online)

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Summary

Washed rabbit platelets resuspended in plasma anticoagulated with the disodium magnesium salt of EDTA (magnesium EDTA) aggregate spontaneously. Analysis of this phenomenon has demonstrated that this aggregation is caused by the slightly elevated concentrations of magnesium present in plasmas anticoagulated with this agent. Addition of magnesium ion in appropriate concentrations to platelet-rich-plasma anticoagulated with 0.38% sodium citrate or 0.1% disodium dihydrogen EDTA (sodium EDTA) also produces gross clot formation, an effect which does not occur when very slightly higher concentrations of sodium EDTA are employed. The present studies distinguish between the effects of calcium ion and magnesium ion in the production of both platelet aggregation and clot formation in several commonly employed anticoagulant solutions. They demonstrate that platelet aggregation is produced by magnesium ion alone, that this aggregation is not associated with release of platelet serotonin, and that it is not caused by ADP.