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Thromb Haemost 1967; 17(03/04): 516-531
DOI: 10.1055/s-0038-1654176
Originalarbeiten - Original Articles - Travaux Originaux
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Platelet Aggregation by Magnesium Ion

R. M Des Prez M. D.,
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
R. E Bryant M. D.,
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
J. A Katz
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
,
Th. E Brittingham M. D.
1   Department of Medicine, Vanderbilt University and Veterans Administration Hospital, Nashville, Tennessee
› Author AffiliationsSupported by research grants HE 08399 and AI 03082 from the National Institutes of Health, U. S. Public Health Service.
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Publication Date:
26 June 2018 (online)

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Summary

Washed rabbit platelets resuspended in plasma anticoagulated with the disodium magnesium salt of EDTA (magnesium EDTA) aggregate spontaneously. Analysis of this phenomenon has demonstrated that this aggregation is caused by the slightly elevated concentrations of magnesium present in plasmas anticoagulated with this agent. Addition of magnesium ion in appropriate concentrations to platelet-rich-plasma anticoagulated with 0.38% sodium citrate or 0.1% disodium dihydrogen EDTA (sodium EDTA) also produces gross clot formation, an effect which does not occur when very slightly higher concentrations of sodium EDTA are employed. The present studies distinguish between the effects of calcium ion and magnesium ion in the production of both platelet aggregation and clot formation in several commonly employed anticoagulant solutions. They demonstrate that platelet aggregation is produced by magnesium ion alone, that this aggregation is not associated with release of platelet serotonin, and that it is not caused by ADP.

 

  • References

  • 1 Chaberek S, Martell A. E. Organic sequestering agents. 514-572 John Wiley and Sons; New York: 1959
    Search in Google Scholar
  • 2 Hovig T. The effect of calcium and magnesium on rabbit blood platelet aggregation in vitro . Thrombos. Diathes. haemorrh. (Stuttg.) 12: 179 1964;
    PubMedSearch in Google Scholar
  • 3 Des Prez R. M, Horowitz H. I, Hook E. W. Effects of bacterial endotoxin on rabbit platelets. I. Platelet aggregation and release of platelet factors in vitro . J. exp. Med 114: 857 1961;
    CrossrefPubMedSearch in Google Scholar
  • 4 Born G. V. R. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (Lond.) 194: 927 1962;
    PubMedSearch in Google Scholar
  • 5 O’Brien J. R. Platelet aggregation I. Some effects of the adenosine phosphates, thrombin, and cocaine upon platelet adhesiveness. J. clin. Path 15: 446 1962;
    CrossrefPubMedSearch in Google Scholar
  • 6 Udenfriend S, Weissbach H, Brodie B. B. Assay of serotonin and related metabolites, enzymes, and drugs. Meth. biochem. Anal 6: 95 1958;
    PubMedSearch in Google Scholar
  • 7 Grette K. Studies on the mechanism of thrombin-catalyzed hemostatic reactions in blood platelets. Actaphysiol scand 56 Suppl. 195 1962;
    PubMedSearch in Google Scholar
  • 8 Waalkes T. P, Coburn H. Comparative effects of glycogen and antigen-antibody reactions on serotonin and histamine in the rabbit. Proc. Soc. exp. Biol. (N. Y.) 101: 122 1959;
    CrossrefPubMedSearch in Google Scholar
  • 9 Westerholm B. Observations on 5-hydroxytryptamine and histamine release from rabbit platelets. Acta physiol. scand 63: 257 1965;
    CrossrefPubMedSearch in Google Scholar
  • 10 Waalkes T. P, Coburn H. The role of platelets and the release of serotonin and histamine during anaphylaxis in the rabbit. J. Allergy 30: 394 1959;
    CrossrefPubMedSearch in Google Scholar
  • 11 Haslam R. J. Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids. Nature (Lond.) 202: 765 1964;
    CrossrefPubMedSearch in Google Scholar
  • 12 Sharp A. A. Present status of platelet aggregation. New Engl. J. Med 272: 89 1965;
    CrossrefPubMedSearch in Google Scholar
  • 13 Biologic Handbooks, Blood and Other Body Fluids. Dittmer D. S. editor 35 Federation of American Societies for Experimental Biology, Washington 1961
    PubMedSearch in Google Scholar
  • 14 Mason R. G, LeRoy E. C, Brinkhous K. M. Blood Platelets, Natural Thrombocyte Agglutinating Systems: Thrombocyte agglutinating factors (TAg and TAg’) in Plasma. Johnson S. A, Monto R. A, Rebuen J. W, Horn R. C. editors 111 Henry Ford Hospital Symposium. Little, Brown and Company; Boston: 1961
    Search in Google Scholar
  • 15 Brinkhous K. M, LeRoy E. C, Cornell W. P, Brown R. C, Hazlehurst J. L, Vennart G. P. Macroscopic Studies of Platelet Agglutination ; Nature of Thrombocyte Agglutinating Activity of Plasma. Proc. Soc. exp. Biol. (N. Y.) 98: 379 1958;
    CrossrefPubMedSearch in Google Scholar
  • 16 Mason R. G, LeRoy E. C, Brinkhous K. M. Cation Specificity of Thrombocyte Agglutinating Activity (TAg) of Canine Plasma. Proc. Soc. exp. Biol. (N. Y.) 102: 253 1959;
    CrossrefPubMedSearch in Google Scholar
  • 17 LeRoy E. C, Mason R. G, Brinkhous K. M. Species differences in platelet agglutination in man and in the dog, swine and rabbit. Amer. J. Physiol 199: 183 1960;
    PubMedSearch in Google Scholar
  • 18 Mason R. G, Hocutt E. J, Wagner R. H, Brinkhous K. M. Evolution and Decay of Platelet-Agglutinating Activity in Normal and Pathologic Human Plasmas. J. Lab. clin. Med 59: 645 1962;
    PubMedSearch in Google Scholar
  • 19 Robinson Jr. C. W, Mason R. G, Wagner R. H. Effect of Sulfhydryl Inhibitors on Platelet Agglutinability. Proc. Soc. exp. Biol. (N. Y.) 113: 857 1963;
    CrossrefPubMedSearch in Google Scholar
  • 20 Garvin J. E. Factors affecting the adhesiveness of human leukocytes and platelets in vitro . J. exp. Med 114: 51 1961;
    CrossrefPubMedSearch in Google Scholar
  • 21 Allison Jr. F, Lancaster M. G, Crossthwaite J. L. Studies on the pathogenesis of acute inflammation. V. An assessment of factors that influence in vitro the phagocytic and adhesive properties of leukocytes obtained from rabbit peritoneal exudate. Amer. J. Path 43: 775 1963;
    PubMedSearch in Google Scholar
  • 22 Bryant R, Des Prez R, Van Way M, Rogers D. Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration and phagocytosis on leukocyte migration, adhesiveness, and aggregation. J. exp. Med. (In press.)
    PubMed
  • 23 Huntsman R. G, Htirn B. A. L, Lehmann H. Paradoxical effect of magnesium ions on blood coagulation. Nature (Lond.) 185: 852 1960;
    PubMedSearch in Google Scholar
  • 24 Lundblad R. L, Davie E. W. The stepwise activation of Antihemophilic Factor (Factor VIII) and Stuart Factor (Factor N) during normal blood coagulation. Fed. Proc 24: 512 (Part I) 1965;
    PubMedSearch in Google Scholar