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Thromb Haemost 1965; 13(01): 155-175
DOI: 10.1055/s-0038-1656222
Originalarbeiten — Original Articles — Travaux Originaux
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Kinetic Aspects of the Interaction of Blood Clotting Enzymes

I. Derivation of Basic Formulas
H. C Hemker
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
,
P.W Hemker
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
,
E. A Loeliger
1   Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.
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Publication Date:
27 June 2018 (online)

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Summary

Application of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :

1. Substrate is not present in excess.

2. Only relative measures exist for concentrations of substrate or enzymes.

3. Enzymes and substrates are often added together.

4. Reagents are not pure.

5. Clotting-time is our only measure for clotting-velocity.

Formulas are deduced, which makes it possible to recognize the effect of these complications.

 

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