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Thromb Haemost 1984; 51(02): 145-149
DOI: 10.1055/s-0038-1661046
Original Article
Schattauer GmbH Stuttgart

Platelet-Platelet Recognition During Aggregation: Distinct Mechanisms Determined by the Release Reaction

Galila Agam
The Department of Biology, Ben-Gurion University of the Negev, Beer-Sheva, Israel
,
Avinoam Livne
The Department of Biology, Ben-Gurion University of the Negev, Beer-Sheva, Israel
› Author Affiliations
Further Information

Publication History

Received 02 August 1983

Accepted 19 December 1983

Publication Date:
19 July 2018 (online)

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Summary

Fixed platelets, bearing covalently bound fibrinogen, were previously shown to participate passively in aggregation induced by thrombin or A23187. The bound fibrinogen was specifically required for the interaction. The present study examines the role of the release reaction in controlling the passive participation of the fixed platelets in aggregation. Aggregation of fresh platelets induced by low ADP concentration (≤2 μM) was not augmented by the fixed platelets, but was augmented when higher concentrations of ADP were employed. Fixed platelets failed to enhance aggregation of aspirin-treated fresh platelets induced by 10 μM ADP; the augmentation capability was reconstituted by a supernatant fraction from platelets activated by 10 μM ADP in the presence of EDTA. The binding of soluble fibrinogen to activated fresh platelets occurred regardless of the release reaction, but the interaction of fragmented fixed platelets bearing bound fibrinogen with aggregating fresh platelets was apparent only when release took place. It is concluded that fibrinogen covalently bound to fixed platelets is selectively recognized by released compound(s).

Keywords

Platelet recognition - Aggregation - ADP - Release reaction
 

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