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Planta Med 2000; 66(8): 724-727
DOI: 10.1055/s-2000-9876
Original Paper
© Georg Thieme Verlag Stuttgart · New York

Peroxidase Activity in Aloe barbadensis Commercial Gel: Probable Role in Skin Protection

Alberto Esteban, José  M. Zapata, Leonardo Casano, Mercedes Martín, Bartolomé Sabater*
  • Departamento de Biología Vegetal, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain
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Publication History

Publication Date:
31 December 2000 (online)

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Abstract

A basic peroxidase (EC 1.11.1.7) (pI around 9.0) has been identified in commercial gel of Aloe barbadensis. In vivo, the activity is localised in the vascular system of inner aqueous leaf parenchyma. Some relevant properties of this basic peroxidase of Aloe have been investigated in leaf extract and in commercial gel where it is notably stable. The acid optimum pH (5.0) for activity and the low K M for H2O2 (0.14 mM) suggest that, when topically applied, Aloe peroxidase may scavenge H2O2 in skin surface.

Abbreviations

4-MN:4-methoxy-α-naphthol BSA:bovine serum albumin EGTA:ethylene glycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid HQ:p-hydroquinone IEF:isoelectrofocusing IALP:inner aqueous leaf parenchyma PVDF:polyvinylidene difluoride ROS:reactive oxygen species pI:isoelectric pH

Key words

Ageing - Aloe barbadensis - Asphodelaceae - peroxidase - reactive oxygen species - skin photo-damage

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Dr. Bartolomé Sabater

Departamento de Biología Vegetal Universidad de Alcalá

Alcalá de Henares

28871 Madrid

Spain

Email: bvsabater@alcala.es

Phone: +34 91 885 5066

Fax: +34 91 885 4934