A Secoisopimarane Diterpenoid from Salvia cinnabarina Inhibits Rat Urinary Bladder Contractility in vitro

Abstract

We have evaluated the effect of 3,4-secoisopimar-4(18),7,15-triene-3-oic acid (compound 1), isolated from the aerial parts of Salvia cinnabarina, on the contractile response elicited by electrical field stimulation (EFS) in the rat isolated urinary bladder. Compound 1 (10 ^{- 7} - 10 ^{- 4} M) produced a concentration-dependent inhibition of the EFS contractile response without modifying the contractions produced by exogenous acetylcholine (10 ^{- 6} M). A number of antagonists/inhibitors including a combination of atropine (10 ^{- 6} M), phentolamine (10 ^{- 6} M), propranolol (10 ^{- 6} M) and hexamethonium (10 ^{- 4} M), the NK₁ receptor antagonist SR140333 (10 ^{- 7} M) plus the NK₂ receptor antagonist SR48968 (10 ^{- 6} M), naloxone (10 ^{- 6} M), verapamil (10 ^{- 7} M), capsazepine (10 ^{- 5} M) and the CB₁ receptor antagonist SR141716A (10 ^{- 6} M) did not modify the inhibitory effect of compound 1. However, the nitric oxide (NO) synthase inhibitor L-NAME (3 × 10 ^{- 4} M), significantly reduced the inhibitory effect of compound 1. It is concluded that compound 1 inhibits rat bladder contractility with a mechanism involving, at least in part, NO production.

References

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